Pig hemoglobin (Hb) ELISA Kit

说明书下载

产品详情

产品描述：

猪血红蛋白(Hb)酶联免疫试剂盒（CSB-EQ027276PI）为竞争法ELISA试剂盒，定量检测血清、血浆、RBC裂解物.样本中的Hb含量。Hb即血红蛋白，是红细胞内运输氧的特殊蛋白质。其与氧气结合和释放对维持机体氧供至关重要。在研究机制上，常关注其结构与功能改变，如与疾病相关的变异影响携氧能力，也有基于它开发药物改善氧运输等方面的研究。试剂盒检测范围为0.625 μg/mL-40 μg/mL，适用于科研领域评估红细胞功能及血红蛋白代谢相关研究；可用于营养学研究中评估饲料中铁元素吸收效率、生理学实验中的贫血模型构建，或毒理学实验中红细胞损伤程度的分析，为实验室提供稳定、高效的检测方案，满足多种样本类型的血红蛋白定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

Hb
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, lysate for RBC.
检测范围：

0.625 μg/mL-40 μg/mL
灵敏度：

0.312 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig Hb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-105
1:2	Average %	92
	Range %	86-100
1:4	Average %	95
	Range %	89-99
1:8	Average %	95
	Range %	87-105

回收率：

The recovery of pig Hb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-99
Plasma (n=5)	96	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.