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  4. Pig interleukin 4,IL-4 ELISA Kit

Pig interleukin 4,IL-4 ELISA Kit

文献引用 (4) 说明书下载
  • 中文名称：
    猪白介素4(IL-4)酶联免疫试剂盒
  • 货号：
    CSB-E06785p
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO猪白介素4（IL-4）酶联免疫吸附检测试剂盒（货号CSB-E06785p），采用双抗体夹心法原理实现定量检测，适用于分析血清、血浆、细胞培养上清液及组织匀浆等样本中的IL-4蛋白水平。IL-4作为Th2型细胞因子，在调节体液免疫、抗寄生虫免疫应答及过敏反应中发挥重要作用，是研究猪免疫调控机制的关键分子。试剂盒检测灵敏度高，线性范围为6.25-400 pg/mL，最低可精确检测至3.12 pg/mL，标准品与样本均设置双复孔检测以保证数据可靠性。实验全程约需4.5小时，包含预包被捕获抗体的96孔板、生物素标记检测抗体及标准品等核心组分，试剂盒保存于2-8℃条件下，开封后建议标准品分装冻存。该产品可广泛应用于猪免疫相关疾病模型构建、疫苗效力评估、炎症机制研究等科研领域，为探索IL-4在感染性疾病、自身免疫性疾病及过敏性反应中的作用提供可靠工具，尤其适用于比较医学研究和畜牧兽医领域的免疫学实验需求。
  • 别名：
    IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
  • 缩写：
    IL4
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %82-91
    1:2Average %90
    Range %85-95
    1:4Average %89
    Range %84-93
    1:8Average %96
    Range %92-101
  • 回收率：
    The recovery of pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10095-105
    EDTA plasma (n=4)9286-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.719 2.652 2.686 2.492
    2001.962 1.861 1.912 1.718
    1001.213 1.204 1.209 1.015
    500.748 0.689 0.719 0.525
    250.551 0.527 0.539 0.345
    12.50.355 0.374 0.365 0.171
    6.250.295 0.272 0.284 0.090
    00.192 0.196 0.194 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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IL4 Proteins

靶点详情

  • 功能：
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
  • 基因功能参考文献：
    1. IL-4- and IL-10-producing invariant NKT cells inhibit the Th1 cell response, but not the Th17 cell response PMID: 21572032
    2. IL-4 induced activation of Akt/SREBP-1/lipid biosynthesis in EC, resulting in protection against membrane attack complex and melittin, in association with mitochondrial protection. PMID: 20017192
    3. There was no significant difference of IL-4 production between fresh and frozen peripheral blood mononuclear cells. PMID: 19766617
    4. The endothelium and subendothelium of porcine iliac arteries that are transduced with recombinant pig IL-4 are effectively protected from complement-dependent immediate injury after perfusion with human blood. PMID: 17082655
    5. unlike in humans and mice, porcine IL-4 blocks antibody and IL-6 secretion and suppresses antigen-stimulated proliferation of B cells PMID: 18761033

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-4/IL-13 family
  • 数据库链接：

    KEGG: ssc:397225

    STRING: 9823.ENSSSCP00000015193

    UniGene: Ssc.15837

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