Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于科研领域的兔Ⅰ型胶原(Col Ⅰ)酶联免疫检测试剂盒（货号：CSB-E06858Rb），采用双抗体夹心ELISA原理实现定量分析，可精准检测血清、血浆及组织匀浆样本中Ⅰ型胶原蛋白的含量，检测灵敏度可达0.156 ng/mL，线性范围覆盖0.156-10 ng/mL。Ⅰ型胶原作为细胞外基质核心成分，广泛分布于皮肤、骨骼及肌腱等结缔组织，其表达水平与组织纤维化、创伤修复及器官硬化等病理生理过程密切相关。本试剂盒通过高特异性抗体对实现靶标捕获与信号放大，适用于体外研究胶原代谢调控机制、评估纤维化疾病模型进展或探索组织工程材料中胶原沉积规律等科研场景。实验操作兼容常规酶标仪检测，提供标准品及预包被板条，支持批量样本分析。需注意样本采集后需按规范预处理，避免反复冻融，组织匀浆建议离心取上清检测以确保数据准确性。本产品严格验证批次稳定性，为科研工作者提供可靠的胶原蛋白定量工具。
别名：

Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
缩写：

Col Ⅰ
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	90
	Range %	85-96
1:4	Average %	88
	Range %	84-92
1:8	Average %	104
	Range %	100-109

回收率：

The recovery of rabbit Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	99	94-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.