Rabbit Estradiol,E2 ELISA Kit

产品详情

文献引用 (1) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔子雌二醇(E2)酶联免疫试剂盒（CSB-E06915Rb）为竞争法ELISA试剂盒，定量检测血清、血浆样本中的E2含量。E2 是重要靶点，在多种生物过程如病毒复制、细胞周期调控等有作用。研究机制方面，围绕其与配体结合模式、信号传导通路开展，探索阻断其活性以抑制病毒感染、肿瘤生长等，有望为相关疾病治疗提供新思路和策略。试剂盒检测范围为62.5 pg/mL-1000 pg/mL，适用于科研场景中兔类生殖内分泌机制研究、环境激素干扰评估、药理学实验的激素动态监测，以及畜牧领域繁殖性能相关基础研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

E2
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma
检测范围：

62.5 pg/mL-1000 pg/mL
灵敏度：

62.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	87
	Range %	84-90
1:4	Average %	87
	Range %	83-91
1:8	Average %	96
	Range %	93-99

回收率：

The recovery of rabbit E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	91-97
EDTA plasma (n=4)	101	98-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.