Rabbit Interleukin 4,IL-4 ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于科研领域的兔源白介素4（IL-4）酶联免疫检测试剂盒（货号：CSB-E06902Rb），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中IL-4蛋白水平。IL-4作为Th2细胞分泌的关键细胞因子，在B淋巴细胞增殖分化、抗体类型转换及抗寄生虫免疫中发挥核心调控作用。试剂盒具备0.625-40 pg/mL的线性检测范围，灵敏度达0.312 pg/mL，可在3小时内完成实验流程，配套提供预包被96孔板、标准品、生物素标记检测抗体及显色底物，操作时仅需样本前处理、孵育结合及显色读数三步。该试剂盒适用于动物模型中的Th2型免疫应答研究、炎症性疾病机制探索或感染过程中细胞因子动态监测，兼容多种生物学样本类型，组分经优化可避免常见交叉反应，所有试剂需在-20°C避光保存，开封后标准品与酶结合物建议分装冻存以确保6个月内性能稳定。
别名：

IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写：

IL4
Uniprot No.：

Q9MZR8
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 pg/mL-40 pg/mL
灵敏度：

0.156 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	100
	Range %	91-105
1:4	Average %	97
	Range %	92-105
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
亚细胞定位：

Secreted.
蛋白家族：

IL-4/IL-13 family
数据库链接：

KEGG: ocu:100302454

STRING: 9986.ENSOCUP00000018901

UniGene: Ocu.2462