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  4. Rabbit basic fibroblast growth factor,bFGF ELISA Kit

Rabbit basic fibroblast growth factor,bFGF ELISA Kit

说明书下载
  • 中文名称：
    兔子碱性成纤维细胞生长因子(bFGF)酶联免疫试剂盒
  • 货号：
    CSB-E06938Rb
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO专用于定量检测兔血清、血浆及组织匀浆样本中碱性成纤维细胞生长因子（bFGF）的酶联免疫试剂盒（货号：CSB-E06938Rb），基于双抗体夹心法原理设计，检测灵敏度达7.8 pg/mL，线性范围为7.8-500 pg/mL。bFGF是一种多功能细胞因子，在血管生成、创伤修复及胚胎发育中发挥关键调控作用，其表达水平与多种生理病理过程密切相关。试剂盒采用高特异性配对抗体，可精准捕获目标蛋白并通过酶标系统实现定量分析，适用于体外培养细胞模型、动物组织样本或体液样本的bFGF含量检测，为研究组织再生机制、肿瘤微环境调控或炎症反应等科研领域提供可靠工具。实验操作全程约需4.5小时，配套提供预包被板条、标准品及检测所需试剂，建议实验前通过梯度稀释将样本浓度调整至线性范围内以确保检测准确性。本产品仅限科学研究使用。
  • 别名：
    FGF2 ELISA Kit; Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit; Fragment ELISA Kit
  • 缩写：
    FGF2
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %84-90
    1:2Average %104
    Range %100-108
    1:4Average %85
    Range %81-89
    1:8Average %99
    Range %95-102
  • 回收率：
    The recovery of rabbit bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8380-86
    EDTA plasma (n=4)9794-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5001.863 1.812 1.838 1.679
    2501.699 1.598 1.649 1.490
    1251.428 1.436 1.432 1.273
    62.51.115 1.168 1.142 0.983
    31.20.804 0.831 0.818 0.659
    15.60.563 0.574 0.569 0.410
    7.80.362 0.372 0.367 0.208
    00.159 0.158 0.159
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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FGF2 Proteins

靶点详情

  • 功能：
    Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
  • 亚细胞定位：
    Secreted. Nucleus.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 数据库链接：

    STRING: 9986.ENSOCUP00000000416

    UniGene: Ocu.1874

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