Rabbit interleukin 12 (IL-12/P70) ELISA kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔白介素12(IL-12/P70)酶联免疫试剂盒（CSB-EQ027628RB）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL12/P70含量。IL12/P70是重要的免疫靶点。它由p35和p40亚基组成，在调节免疫细胞功能、促进Th1细胞分化等方面发挥关键作用。其研究机制主要聚焦于调控免疫应答相关信号通路。深入研究它有望为免疫相关疾病治疗提供新策略。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品适用于实验动物模型的免疫调控研究、感染性疾病机制探索、疫苗效力评估及炎症相关科研项目，为研究IL-12/P70在免疫微环境中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IL12/P70, Interleukin-12/P70
缩写：

IL-12/P70
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-95
1:2	Average %	90
	Range %	83-99
1:4	Average %	95
	Range %	92-102
1:8	Average %	93
	Range %	86-99

回收率：

The recovery of rabbit IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-110
EDTA plasma (n=4)	97	89-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.