Rabbit monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA kit
产品详情
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产品描述：
兔子单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)酶联免疫试剂盒（CSB-E06921Rb）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL2含量。CCL2是趋化因子家族的重要成员。其背景在于它能趋化单核细胞、记忆T淋巴细胞和树突状细胞等至炎症部位。研究机制方面，它主要通过与CCR2等受体结合，激活下游信号通路，参与炎症反应、肿瘤进展等多种病理生理过程。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于体外研究炎症性疾病动物模型、免疫细胞趋化实验或相关信号通路机制探索等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：
CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
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缩写：
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Uniprot No.：
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种属：
Oryctolagus cuniculus (Rabbit)
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样本类型：
serum, plasma, tissue homogenates
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检测范围：
23.5 pg/mL-1500 pg/mL
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灵敏度：
5.87 pg/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Immunology
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 90-100 | |
| 1:2 | Average % | 95 | |
| Range % | 91-100 | |
| 1:4 | Average % | 95 | |
| Range % | 89-101 | |
| 1:8 | Average % | 97 | |
| Range % | 91-100 | |
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回收率：
| The recovery of rabbit MCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 91-99 | |
| EDTA plasma (n=4) | 98 | 94-104 | |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 1500 | 2.488 | 2.501 | 2.495 | 2.322 | | | 750 | 1.985 | 1.999 | 1.992 | 1.819 | | | 375 | 1.483 | 1.471 | 1.477 | 1.304 | | | 187.5 | 0.938 | 0.942 | 0.940 | 0.767 | | | 94 | 0.574 | 0.584 | 0.579 | 0.406 | | | 47 | 0.382 | 0.396 | 0.389 | 0.216 | | | 23.5 | 0.251 | 0.253 | 0.252 | 0.079 | | | 0 | 0.174 | 0.172 | 0.173 | | |
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数据处理：
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货期：
3-5 working days
靶点详情
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功能：
Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
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基因功能参考文献：
- Data show that peripheral blood endothelial progenitor cells can increase the expressions of chemokines CCL2 and CXCL12 and promote the migration of enhanced green fluorescent protein-positive bone marrow stromal cells to bone defect site. PMID: 26728372
- Data show that the serum levels of mast cell tryptase, monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) decreased significantly in Dachengqi decoction treatment group. PMID: 25270202
- measurements of CCL2 and macrophages can serve as new inflammatory biomarkers to evaluate the safety of promising novel microbicides for prevention of HIV. PMID: 19929801
- Expression of MCP-1 was successfully inhibited by transfecting MCP-1 siRNA expression plasmid to the carotid artery, and the progression of atherosclerosis was restricted by RNAi-mediated silencing of MCP-1 expression. PMID: 19467827
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亚细胞定位：
Secreted.
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蛋白家族：
Intercrine beta (chemokine CC) family
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数据库链接：
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