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  4. Rat Bone morphogenetic protein 4,BMP-4 ELISA Kit

Rat Bone morphogenetic protein 4,BMP-4 ELISA Kit

说明书下载
  • 中文名称：
    大鼠骨成型蛋白4(BMP-4)酶联免疫试剂盒
  • 货号：
    CSB-E04511r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠骨成型蛋白4（BMP-4）酶联免疫检测试剂盒（货号：CSB-E04511r），采用双抗体夹心法原理进行定量检测，可精准分析血清、血浆及组织匀浆样本中BMP-4的浓度，检测范围为7.8-500 pg/mL，灵敏度达7.8 pg/mL，整个检测流程约3小时即可完成。BMP-4作为转化生长因子β（TGF-β）超家族成员，在骨骼发育、胚胎形成、细胞分化及组织修复等生理过程中发挥关键调控作用，其表达水平变化与骨骼代谢异常、组织再生等研究密切相关。本试剂盒适用于科研领域中对BMP-4功能机制的探索，例如骨骼发育动态研究、再生医学中组织修复模型的分子机制解析、胚胎发育过程中形态发生调控的定量分析，以及相关疾病模型的分子标志物检测。试剂盒提供预包被微孔板及标准化试剂组分，操作便捷且重复性良好，可为体外实验提供高特异性检测方案，满足基础研究中对BMP-4蛋白表达的定量需求。
  • 别名：
    Bmp4 ELISA Kit; Bmp-4 ELISA Kit; Dvr-4Bone morphogenetic protein 4 ELISA Kit; BMP-4 ELISA Kit; Bone morphogenetic protein 2B ELISA Kit; BMP-2B ELISA Kit
  • 缩写：
    BMP4
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %98
    Range %94-105
    1:2Average %91
    Range %85-97
    1:4Average %86
    Range %82-90
    1:8Average %94
    Range %90-100
  • 回收率：
    The recovery of rat BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-93
    EDTA plasma (n=4)9287-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1OD2AverageCorrected
    5002.514 2.651 2.583 2.468
    2501.867 1.797 1.832 1.717
    1251.279 1.315 1.297 1.182
    62.50.885 0.842 0.864 0.749
    31.20.513 0.538 0.526 0.411
    15.60.294 0.289 0.292 0.177
    7.80.201 0.213 0.207 0.092
    00.112 0.118 0.115
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical BMP pathways such as ERK/MAP kinase, PI3K/Akt, or SRC cascades. For example, induces SRC phosphorylation which, in turn, activates VEGFR2, leading to an angiogenic response.
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    TGF-beta family
  • 数据库链接：

    STRING: 10116.ENSRNOP00000012957

    UniGene: Rn.10318

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