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  4. Rat Cardiac Troponin T(cTn-T)ELISA kit

Rat Cardiac Troponin T(cTn-T)ELISA kit

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  • 中文名称：
    大鼠心肌肌钙蛋白T(cTn-T)酶联免疫试剂盒
  • 货号：
    CSB-E16443r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO大鼠心肌肌钙蛋白T（cTn-T）酶联免疫试剂盒（货号：CSB-E16443r），基于双抗体夹心法原理设计，适用于血清、血浆及组织匀浆样本中cTn-T的定量检测。cTn-T是心肌肌钙蛋白复合体的关键亚基，特异分布于心肌细胞中，其释放水平与心肌损伤程度密切相关，是研究心脏病理机制的重要生物标志物。试剂盒检测范围为12.5 pg/mL至800 pg/mL，通过预包被特异性抗体实现高灵敏度检测，实验流程包含标准品稀释、样本孵育、洗涤及显色步骤，配套提供标准品、检测抗体、酶结合物及终止液等完整组分。该试剂盒可广泛应用于心脏疾病动物模型研究，如心肌缺血再灌注损伤、药物心脏毒性评估、心肌炎或心力衰竭机制探索等科研领域，为体外定量分析心肌损伤提供可靠工具。实验需自备酶标仪（450 nm波长）、移液器及离心机等基础设备，建议严格按照操作说明进行样本处理与检测。
  • 别名：
    Tnnt2 ELISA Kit; Troponin T ELISA Kit; cardiac muscle ELISA Kit; TnTc ELISA Kit; Cardiac muscle troponin T ELISA Kit; cTnT ELISA Kit
  • 缩写：
    cTn-T
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 pg/mL-800 pg/mL
  • 灵敏度：
    3.12 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-T in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %82-97
    1:2Average %87
    Range %81-94
    1:4Average %93
    Range %87-100
    1:8Average %95
    Range %89-102
  • 回收率：
    The recovery of rat cTn-T spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9790-105
    EDTA plasma (n=4)9588-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    8002.187 2.102 2.145 1.986
    4001.731 1.641 1.686 1.527
    2001.254 1.204 1.229 1.070
    1000.926 0.879 0.903 0.744
    500.593 0.554 0.574 0.415
    250.302 0.279 0.291 0.132
    12.50.241 0.227 0.234 0.075
    00.165 0.153 0.159 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Tnnt2 Proteins

靶点详情

  • 功能：
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 基因功能参考文献：
    1. Replacement of the functionally corresponding N-terminal end portion of rat fast skeletal cardiac muscle (RfsTnT) into cardiac muscle troponin T (RcTnT), the observed functional differences associate with a sequence variation. PMID: 23357173
    2. L48Q cTnC reduced crossbridge dependence of thin filament activation in cardiac muscle and changes in Ca(2+) sensitivity of force in response to changes in sarcomere length are at least partially dependent on properties of thin filament troponin. PMID: 22865385
    3. cTnC, cTnI, cTnT and cTm are not only present in myofilaments of ventricular cardiomyocytes in culture but are also within their nuclei; significantly, these four proteins appear between days 3 and 5 in both myofilaments and nuclei PMID: 22364878
    4. Early single cTnT measurement correlates with infarct size in rats, and a cutoff value of 5.1 mug/l provides good sensitivity and specificity to predict congestive heart failure with secondary pulmonary hypertension. PMID: 20537565
    5. The time course of cTnT elevation was temporally associated with evidence of increased lipid peroxidation in the rat heart. PMID: 20711602
    6. a novel role for cTnT as a dual-specificity sarcomeric A-kinase anchoring protein PMID: 21056973
    7. Studies show that the rat Tnnt2-rtTA;TetO-Cre mice transgenic line a valuable genetic tool for analysis of spatiotemporal gene function and cardiomyocyte lineage tracing during developmental and postnatal period. PMID: 20014345
    8. The mu-calpain-mediated proteolytic modification of TnT may act as an acute mechanism to adjust muscle contractility under stress conditions. PMID: 16981728
    9. Overexpression of heat shock protein 27 protects against ischaemia/reperfusion-induced cardiac dysfunction via stabilization of troponin I and T. PMID: 18397962
    10. Mdetabolic inhibition of cardiomyocytes induces a parallel release of intact Tnnt3 and its degradation products, starting only after onset of irreversible cardiomyocyte damage. PMID: 18721805
    11. Study demonstrate profound energetic alterations in DEL-TNT hearts, supporting the notion that inefficient cellular ATP utilization contributes to the pathogenesis of HCM. PMID: 19189074

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  • 蛋白家族：
    Troponin T family
  • 数据库链接：

    KEGG: rno:24837

    STRING: 10116.ENSRNOP00000049297

    UniGene: Rn.9965

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