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  4. Rat Cathepsin S(CTSS) ELISA kit

Rat Cathepsin S(CTSS) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    大鼠组织蛋白酶S(CTSS)酶联免疫试剂盒
  • 货号：
    CSB-EL006204RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠组织蛋白酶S(CTSS)酶联免疫试剂盒（CSB-EL006204RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CTSS含量。CTSS（组织蛋白酶S）是一种溶酶体半胱氨酸蛋白酶，参与抗原呈递和细胞外基质重塑。其在多种疾病如皮肤纤维瘤、肝纤维化中发挥作用，成为潜在的治疗靶点。研究显示，CTSS通过调节细胞外基质成分，影响疾病进程。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外实验中的蛋白表达水平监测、疾病机制研究及药物干预效果评价等科研领域，满足细胞实验、动物模型组织分析等多种研究需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Ctss ELISA Kit; Cathepsin S ELISA Kit; EC 3.4.22.27 ELISA Kit
  • 缩写：
    CTSS
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CTSS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %88-98
    1:2Average %97
    Range %93-105
    1:4Average %90
    Range %85-94
    1:8Average %98
    Range %94-102
  • 回收率：
    The recovery of rat CTSS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10398-110
    EDTA plasma (n=4)8480-89
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.563 2.457 2.510 2.395
    5001.997 2.076 2.037 1.922
    2501.378 1.404 1.391 1.276
    1250.877 0.821 0.849 0.734
    62.50.567 0.554 0.561 0.446
    31.250.358 0.331 0.345 0.230
    15.60.227 0.240 0.234 0.119
    00.113 0.117 0.115
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Ctss Proteins

靶点详情

  • 功能：
    Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.
  • 基因功能参考文献：
    1. Findings demonstrate that neuronal reactive oxygen species promote maturation of microglial CatS which facilitates activation of NMDA in the spinal dorsal horn. PMID: 28039043
    2. Inhibition of cathepsin S release is one mechanism that is responsible for purinergic P2X7 antagonist efficacy in neuropathic pain. PMID: 20629190
    3. Activated rat hepatic stellate cells express the class II transactivator, the invariant chain (CD74), the MHC class II molecules, as well as cathepsin S, all of which are known to be responsible for the initial steps of successful antigen presentation. PMID: 17178165
    4. endogenous CatS released by peripheral macrophages contributes to the maintenance of neuropathic hyperalgesia following nerve injury PMID: 17250968
    5. There is an altered expression of cathepsin S and cathepsin X in the spinal cord of a neuropathic pain model; including cellular distribution and pain development. PMID: 18700000
    6. under neuropathic conditions, noxious stimulation of primary afferent fibers induces release of CatS from microglia, which liberates FKN from dorsal horn neurons, thereby contributing to the amplification and maintenance of chronic pain PMID: 19474321

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  • 亚细胞定位：
    Lysosome. Secreted. Cytoplasmic vesicle, phagosome.
  • 蛋白家族：
    Peptidase C1 family
  • 组织特异性：
    Highest levels occur in the ileum followed by spleen, brain, thyroid, ovary and uterus. Low levels are found in the liver, kidney, jejunum and lung with lowest levels in the heart.
  • 数据库链接：

    KEGG: rno:50654

    UniGene: Rn.11347

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