Rat Clara cell protein,CC16 ELISA Kit
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中文名称：
大鼠克拉拉细胞蛋白(CC16)酶联免疫试剂盒
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货号：
CSB-E08681r
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规格：
96T/48T
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价格：
￥3900/￥2500
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其他：
产品详情
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产品描述：
CUSABIO大鼠克拉拉细胞蛋白(CC16)酶联免疫检测试剂盒（货号：CSB-E08681r），基于双抗体夹心法原理实现定量分析，适用于血清、血浆及组织匀浆样本的CC16蛋白检测。CC16是由呼吸道上皮克拉拉细胞分泌的多功能蛋白，具有抗炎、抗氧化及免疫调节特性，在呼吸道疾病病理机制研究中具有重要价值。该试剂盒检测灵敏度高，线性范围为3.12-200 pg/mL，可精准检测微量样本中CC16的表达水平。实验操作采用预包被特异性抗体的96孔板，通过标准曲线定量目标蛋白浓度，配套试剂包含校准品、检测抗体及显色系统，满足标准化检测需求。该产品适用于呼吸道炎症模型、肺损伤机制研究、环境污染物暴露评估等科研领域，为探索CC16在肺部疾病发生发展中的作用、评估药物治疗效果或生物标志物筛选提供可靠工具，检测数据可支持动物实验中的病理生理学分析及分子机制研究，本产品仅用于科学研究。
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别名：
Scgb1a1 ELISA kit; Cc10 ELISA kit; Ugb ELISA kit; UtgUteroglobin ELISA kit; Clara cell phospholipid-binding protein ELISA kit; CCPBP ELISA kit; Clara cells 10 kDa secretory protein ELISA kit; CC10 ELISA kit; PCB-binding protein ELISA kit; Secretoglobin family 1A member 1 ELISA kit
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缩写：
CC16
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Uniprot No.：
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种属：
Rattus norvegicus (Rat)
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样本类型：
serum, plasma, tissue homogenates
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检测范围：
3.12 pg/mL-200 pg/mL
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灵敏度：
0.78 pg/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Others
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CC16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
92-102 |
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| 1:2 |
Average % |
91 |
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| Range % |
85-98 |
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| 1:4 |
Average % |
90 |
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| Range % |
88-93 |
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| 1:8 |
Average % |
100 |
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| Range % |
96-104 |
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回收率：
| The recovery of rat CC16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
87-92 |
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| EDTA plasma (n=4) |
106 |
103-110 |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.147 |
2.235 |
2.191 |
2.089 |
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| 100 |
1.683 |
1.703 |
1.693 |
1.591 |
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| 50 |
1.231 |
1.255 |
1.243 |
1.141 |
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| 25 |
0.885 |
0.902 |
0.894 |
0.792 |
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| 12.5 |
0.502 |
0.528 |
0.515 |
0.413 |
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| 6.25 |
0.343 |
0.359 |
0.351 |
0.249 |
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| 3.12 |
0.218 |
0.236 |
0.227 |
0.125 |
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| 0 |
0.103 |
0.100 |
0.102 |
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数据处理：
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货期：
3-5 working days
靶点详情
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功能：
Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
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基因功能参考文献：
- The expression level of CC16 in lung tissues and BALF decreased in rat lung tissues exposed to mixed air pollutants. PMID: 18303626
- IL-13 induces CCSP expression via an EGFR- and leukocyte-dependent pathway. PMID: 12060562
- Results show that secretory markers such as CFTR, CC10, and CC26 are present in taste cells of rat circumvallate papillae, and their immunoreactivity is expressed, to a different extent, in subsets of taste cells that express alpha-gustducin. PMID: 18156603
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亚细胞定位：
Secreted.
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蛋白家族：
Secretoglobin family
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组织特异性：
Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
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数据库链接：
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