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  4. Rat Collagen Type Ⅰ,Col Ⅰ ELISA Kit

Rat Collagen Type Ⅰ,Col Ⅰ ELISA Kit

文献引用 (20) 说明书下载
  • 中文名称：
    大鼠Ⅰ型胶原(Col Ⅰ)酶联免疫试剂盒
  • 货号：
    CSB-E08084r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO双抗体夹心法ELISA试剂盒，专为大鼠Ⅰ型胶原（Col Ⅰ）的定量检测而设计。Ⅰ型胶原是细胞外基质的主要成分，广泛分布于皮肤、骨骼及肌腱等结缔组织中，其表达水平与组织纤维化、创伤修复及骨代谢等生理病理过程密切相关。试剂盒采用特异性抗体对，可精准检测血清、血浆及组织匀浆样本中的Col Ⅰ蛋白，检测范围为78-5000 pg/mL，操作流程约需4.5小时。试剂盒包含预包被捕获抗体的酶标板、检测抗体、标准品及显色系统，适用于体外实验环境下对大鼠组织修复机制、纤维化疾病模型评估或骨骼代谢相关研究，为科研人员提供灵敏、稳定的检测方案。检测样本需经离心预处理，建议复孔检测以保证数据可靠性，实验全程无需特殊设备，结果可通过酶标仪在450 nm波长下读取。
  • 别名：
    Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
  • 缩写：
    Col Ⅰ
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-88  
    1:2 Average % 89  
    Range % 84-93  
    1:4 Average % 92  
    Range % 87-96  
    1:8 Average % 95  
    Range % 90-99  
  • 回收率：
    The recovery of rat Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 88-98  
    EDTA plasma (n=4) 98 93-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    5000 2.701 2.762 2.732 2.537  
    2500 1.903 1.954 1.929 1.734  
    1250 1.195 1.207 1.201 1.006  
    625 0.802 0.743 0.773 0.578  
    312 0.489 0.466 0.478 0.283  
    156 0.376 0.359 0.368 0.173  
    78 0.258 0.243 0.251 0.056  
    0 0.201 0.189 0.195 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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