Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat Collagen Type Ⅰ,Col Ⅰ ELISA Kit

Rat Collagen Type Ⅰ,Col Ⅰ ELISA Kit

文献引用 (20) 说明书下载
  • 中文名称：
    大鼠Ⅰ型胶原(Col Ⅰ)酶联免疫试剂盒
  • 货号：
    CSB-E08084r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠Ⅰ型胶原(Col Ⅰ)酶联免疫试剂盒（CSB-E08084r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Col Ⅰ含量。Col Ⅰ即Ⅰ型胶原蛋白，是细胞外基质的主要成分。其背景在于它广泛存在于多种组织，对维持组织正常结构和功能起重要作用。研究机制方面，围绕其在组织纤维化、肿瘤微环境等过程中的合成、降解及信号传导途径展开，旨在寻找相关疾病的治疗靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于体外实验环境下对大鼠组织修复机制、纤维化疾病模型评估或骨骼代谢相关研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
  • 缩写：
    Col Ⅰ
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-88  
    1:2 Average % 89  
    Range % 84-93  
    1:4 Average % 92  
    Range % 87-96  
    1:8 Average % 95  
    Range % 90-99  
  • 回收率：
    The recovery of rat Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 88-98  
    EDTA plasma (n=4) 98 93-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    5000 2.701 2.762 2.732 2.537  
    2500 1.903 1.954 1.929 1.734  
    1250 1.195 1.207 1.201 1.006  
    625 0.802 0.743 0.773 0.578  
    312 0.489 0.466 0.478 0.283  
    156 0.376 0.359 0.368 0.173  
    78 0.258 0.243 0.251 0.056  
    0 0.201 0.189 0.195 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

显示更多
收起更多

产品评价

相关产品

ELISA kits

Most popular with customers

产品中心
特别关注


X