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  4. Rat Complement 3,C3 ELISA Kit

Rat Complement 3,C3 ELISA Kit

文献引用 (5) 说明书下载
  • 中文名称：
    大鼠补体蛋白3(C3)酶联免疫试剂盒
  • 货号：
    CSB-E08666r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO大鼠补体蛋白3（C3）酶联免疫检测试剂盒（货号：CSB-E08666r），基于竞争法原理实现定量分析，适用于血清、血浆及组织匀浆样本的检测。补体蛋白C3作为补体系统的核心成分，在先天免疫中发挥关键作用，参与调理吞噬、免疫复合物清除及炎症反应调控，是研究免疫相关机制的重要生物标志物。试剂盒检测范围为0.625-40 μg/mL，通过预包被特异性抗体与样本中C3蛋白竞争结合标记物，实现精准定量，灵敏度满足低浓度样本检测需求。该产品可广泛应用于科研领域，包括免疫调控机制研究、炎症性疾病动物模型分析、感染或自身免疫病理过程中补体激活状态的动态监测，以及药理学实验中药物对补体系统影响的评估。其兼容多种样本类型的特点，尤其适用于比较不同组织或体液样本中C3表达水平的变化，为探索补体相关通路提供可靠工具。
  • 别名：
    C3Complement C3 [Cleaved into: Complement C3 beta chain; C3-beta-c ELISA kit; C3bc ELISA kit; Neutrophil chemotactic factor-2 ELISA kit; ENCF-2); Complement C3 alpha chain; C3a anaphylatoxin ELISA kit; Neutrophil chemotactic factor-1 ELISA kit; ENCF-1); Acylation stimulating protein ELISA kit; ASP ELISA kit; C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2] ELISA kit
  • 缩写：
    C3
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 μg/mL-40 μg/mL
  • 灵敏度：
    0.156 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 84  
    Range % 81-89  
    1:200 Average % 92  
    Range % 87-96  
    1:400 Average % 101  
    Range % 97-106  
    1:800 Average % 87  
    Range % 84-90  
  • 回收率：
    The recovery of rat C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-96  
    EDTA plasma (n=4) 95 89-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    40 0.085 0.083 0.084    
    20 0.173 0.182 0.178    
    10 0.308 0.321 0.315    
    5 0.576 0.608 0.592    
    2.5 0.983 0.961 0.972    
    1.25 1.462 1.465 1.464    
    0.625 1.825 1.862 1.844    
    0 2.553 2.567 2.560    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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C3 antibodies

C3 Proteins

靶点详情

  • 功能：
    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.; Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. In chronic inflammation, acts as a chemoattractant for neutrophils.; Acts as a chemoattractant for neutrophils in chronic inflammation.; adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2.
  • 基因功能参考文献：
    1. These data suggest that locally produced C3 is an important prosurvival mechanism in pancreatic beta-cells under a proinflammatory assault. PMID: 28582497
    2. Inhibition of C3 might improve the reactivity of superior mesenteric arteries to norepinephrine during hemorrhagic shock possibly through the downregulation of NO, ET1, TNF-alpha and reactive oxygen radicals. PMID: 26687560
    3. Recruited microglia/monocytes contribute to activation of complement in the aging retina, through local expression of C3 mRNA. PMID: 24705166
    4. The C3 and C3a receptor system may primarily be involved in the pathogenesis of renal remodeling in hypertensive rats. PMID: 23713944
    5. The expression of the complement component C3f and the complement component 4A have potential for use in post-transplant diagnosis of rejection. PMID: 23613499
    6. Abnormal activation of complement C3 in the spinal dorsal horn is closely associated with progression of neuropathic pain. PMID: 23588254
    7. The retinal irradiation with 670-nm light substantially reduces the propagation of complement3 in the retina following light-induced degeneration. PMID: 23181358
    8. Early response to blast injury involves activation of complement and TNFalpha, correlating with hippocampus and cerebral cortex immune-inflammatory damage. PMID: 22537900
    9. Carboxypeptidase B2 and complement C3 were more abundant in the cerebrospinal fluid of experimental autoimmune encephalomyelitis rats that did not respond to minocyline treatment. PMID: 22768796
    10. Increase of C3 expression occurs in light damaged retina leading to retinal degeneration. PMID: 22183312
    11. C3 and C4 may be involved in the immunopathology of allergic rhinitis. PMID: 17345707
    12. Perivascular adipose tissue-derived C3 stimulated adventitial fibroblast migration and differentiation via the c-Jun N-terminal kinase pathway and may contribute to adventitial remodeling in a deoxycorticosterone acetate-salt hypertensive model. PMID: 20864665
    13. downregulation in microglia while differentiating into ramified cells PMID: 12137932
    14. complement C3 expression is regulated by the bile acid receptor FXR PMID: 15590640
    15. Increased STAT-1, IRF-1 and complement component C3 in several models of liver transplant tolerance suggests that the STAT-1/IRF-1 apoptotic pathway and C3 may be involved in the tolerogenic mechanism. PMID: 16555329
    16. Complement C1 Inactivator Proteins, in addition to inhibition of the systemic complement activation, prevents myocardial cell injury via a direct inhibitory role in the local myocardial C3 activity PMID: 16996480
    17. C3a and C5a mediate distinct effects on blood pressure and circulating polymorphonuclear leukocytes in the rat. PMID: 19285573
    18. role for C3 in influencing the level of expression of CR3 by modulating the transcript levels encoding the CD11b alpha integrin protein PMID: 19710459

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  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    KEGG: rno:24232

    STRING: 10116.ENSRNOP00000066885

    UniGene: Rn.11378

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