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Rat Corticosterone,CORT ELISA Kit

  • 中文名称:
    大鼠皮质酮(CORT)酶联免疫试剂盒
  • 货号:
    CSB-E07014r
  • 规格:
    96T/48T
  • 价格:
    ¥3900/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO大鼠皮质酮/肾上腺酮(CORT)酶联免疫检测试剂盒(货号:CSB-E07014r),采用竞争法原理实现定量检测,适用于血清、血浆及组织匀浆样本中CORT含量的精准分析。CORT作为大鼠体内主要的糖皮质激素,参与调节应激反应、能量代谢和免疫应答等生理过程,是研究下丘脑-垂体-肾上腺轴(HPA轴)功能的重要指标。该试剂盒检测灵敏度达0.2 ng/mL,线性范围为0.2-40 ng/mL,可覆盖生理及病理状态下大鼠样本中CORT的典型浓度。其标准化操作流程支持科研人员开展神经内分泌机制研究、慢性应激模型评估、代谢综合征相关实验及药物干预效果验证等领域的探索。试剂盒采用预包被板条和即用型试剂设计,配套完整的标准品和检测缓冲体系,确保实验的高重复性与稳定性。通过特异性抗体与样本中CORT分子的竞争性结合反应,结合显色系统的光密度变化,可准确反映目标物的浓度水平,为行为学实验、神经药理学研究和激素调控机制探索提供可靠的数据支持。
  • 别名:
    N/A
  • 缩写:
    CORT
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.2 ng/mL-40 ng/mL
  • 灵敏度:
    0.1 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-91  
    1:2 Average % 90  
    Range % 85-95  
    1:4 Average % 97  
    Range % 92-103  
    1:8 Average % 105  
    Range % 100-112  
  • 回收率:
    The recovery of rat CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    40 0.138 0.132 0.135    
    10 0.239 0.258 0.249    
    3.2 0.504 0.509 0.507    
    0.8 0.939 1.009 0.974    
    0.2 1.419 1.424 1.422    
    0 2.154 2.159 2.157    
  • 数据处理:
  • 货期:
    3-5 working days

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靶点详情

  • 最新研究进展:
    CORT是一种重要的激素,可以影响多种生理过程,如免疫和应激反应。最近的研究表明,CORT可以通过影响免疫细胞的活动来调节免疫反应。例如,在炎症过程中,CORT可以抑制炎症介质的产生,从而缓解炎症反应。此外,CORT也被认为是抑制免疫系统过度激活的重要因素之一。最近的研究还表明,CORT可能在多种神经系统疾病中具有治疗潜力,例如抑郁症和焦虑症等。