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  4. Rat Cytochrome c(CYCS) ELISA kit

Rat Cytochrome c(CYCS) ELISA kit

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  • 中文名称：
    大鼠细胞色素C(CYCS)酶联免疫试剂盒
  • 货号：
    CSB-EL006328RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠细胞色素C(CYCS)酶联免疫试剂盒（CSB-EL006328RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYCS含量。CYCS 即细胞色素 c，是位于线粒体内膜外侧的水溶性蛋白。它在细胞呼吸的电子传递链中发挥关键作用。研究发现，其从线粒体释放到细胞质会激活凋亡相关蛋白，进而启动细胞凋亡机制，在细胞死亡调控等研究领域备受关注。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于大鼠模型中CYCS相关的基础研究，例如线粒体功能评估、凋亡信号通路探索、药物诱导的细胞损伤研究或心血管疾病模型的分子机制分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CycsCytochrome c ELISA Kit; somatic ELISA Kit
  • 缩写：
    CYCS
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CYCS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-94
    1:2Average %89
    Range %85-96
    1:4Average %92
    Range %90-95
    1:8Average %91
    Range %87-96
  • 回收率：
    The recovery of rat CYCS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-96
    EDTA plasma (n=4)9084-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.581 2.458 2.520 2.427
    25002.234 2.186 2.210 2.117
    12501.638 1.621 1.630 1.537
    6251.103 1.097 1.100 1.007
    3120.692 0.672 0.682 0.589
    1560.393 0.386 0.390 0.297
    780.236 0.223 0.230 0.137
    00.094 0.092 0.093 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Cycs Proteins

靶点详情

  • 功能：
    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 亚细胞定位：
    Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.
  • 蛋白家族：
    Cytochrome c family
  • 组织特异性：
    Found in embryos and in adult liver and heart.
  • 数据库链接：

    KEGG: rno:100363502

    STRING: 10116.ENSRNOP00000041521

    UniGene: Rn.161841

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