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Rat Epinephrine/Adrenaline,EPI ELISA Kit

  • 中文名称:
    大鼠肾上腺素(EPI)酶联免疫试剂盒
  • 货号:
    CSB-E08678r
  • 规格:
    96T/48T
  • 价格:
    ¥3900/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO大鼠肾上腺素(EPI)酶联免疫检测试剂盒(货号:CSB-E08678r),适用于定量分析血清、血浆及组织匀浆样本中EPI含量,检测灵敏度为1 pg/mL,线性范围为1-200 pg/mL。肾上腺素作为关键的儿茶酚胺类激素和神经递质,广泛参与机体应激反应、心血管功能调节及能量代谢等生理过程。本试剂盒基于竞争法原理设计,样本中的EPI与预先包被的标准品竞争结合特异性抗体,通过显色反应强度反比推算目标物浓度,操作流程包含样本预处理、孵育、洗涤及比色测定等步骤,试剂盒提供预包被微孔板、系列浓度标准品、检测抗体、显色底物及终止液等完整组分,3.5小时内即可完成检测。该产品可满足神经生物学、药理学及内分泌学等领域的基础研究需求,例如探索应激状态下激素释放机制、心血管疾病模型中交感神经活性评估,或代谢综合征相关动物实验的EPI动态监测,为科研人员提供稳定可靠的定量分析工具。
  • 缩写:
    EPI
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1 pg/mL-200 pg/mL
  • 灵敏度:
    0.5 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 90-103  
    1:2 Average % 90  
    Range % 85-96  
    1:4 Average % 94  
    Range % 89-99  
    1:8 Average % 85  
    Range % 80-90  
  • 回收率:
    The recovery of rat EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 91 85-96  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.203 0.202 0.203    
    50 0.378 0.351 0.365    
    16 0.581 0.537 0.559    
    4 0.945 1.012 0.979    
    1 1.416 1.493 1.455    
    0 2.241 2.367 2.304    
  • 数据处理:
  • 货期:
    3-5 working days

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靶点详情

  • 最新研究进展:
    EPI是一种激素,主要由肾上腺分泌,具有增强心脏功能、提高血糖水平等生理作用。最新研究表明,EPI与多种疾病有关,如心血管疾病、代谢综合征、糖尿病等。一些研究还表明,EPI还可能参与肿瘤的发生和发展。未来的研究将继续探讨EPI在疾病中的作用机制,以及开发针对EPI的治疗方法。