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  4. Rat Glutathione Peroxidase (GSH-PX) ELISA kit

Rat Glutathione Peroxidase (GSH-PX) ELISA kit

文献引用 (43) 说明书下载
  • 中文名称：
    大鼠谷胱甘肽过氧化物酶(GSH-PX)酶联免疫试剂盒
  • 货号：
    CSB-E12146r
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠谷胱甘肽过氧化物酶(GSH-PX)酶联免疫试剂盒（CSB-E12146r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPX1含量。GPX1是重要靶点。它是谷胱甘肽过氧化物酶家族成员，能催化还原型谷胱甘肽变成氧化型谷胱甘肽，清除体内过氧化物和自由基，保护细胞免受氧化损伤。在多种疾病尤其癌症、神经退行性疾病研究中，其作用机制是研究热点。试剂盒检测范围为12.5 mIU/mL-800 mIU/mL，适用于多种科研场景，包括但不限于评估氧化应激模型（如肝脏疾病、神经退行性病变等）中酶活性变化、药物或环境因素对抗氧化系统的影响研究，以及代谢性疾病相关机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Gpx1Glutathione peroxidase 1 ELISA Kit; GPx-1 ELISA Kit; GSHPx-1 ELISA Kit; EC 1.11.1.9 ELISA Kit; Cellular glutathione peroxidase ELISA Kit
  • 缩写：
    GPX1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 mIU/mL-800 mIU/mL
  • 灵敏度：
    3.12 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 92  
    Range % 85-100  
    1:400 Average % 97  
    Range % 94-101  
    1:800 Average % 95  
    Range % 90-99  
    1:1600 Average % 93  
    Range % 87-98  
  • 回收率：
    The recovery of rat GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 86-100  
    EDTA plasma (n=4) 100 95-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected  
    800 2.251 2.299 2.275 2.119  
    400 1.845 1.834 1.840 1.684  
    200 1.301 1.324 1.313 1.157  
    100 0.892 0.876 0.884 0.728  
    50 0.553 0.590 0.572 0.416  
    25 0.395 0.381 0.388 0.232  
    12.5 0.251 0.234 0.243 0.087  
    0 0.155 0.157 0.156 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Gpx1 Proteins

靶点详情

  • 最新研究进展：
    GPX1是一种谷胱甘肽过氧化物酶，可以清除细胞内的过氧化氢等活性氧，具有抗氧化和细胞保护作用。最新研究表明，GPX1在多种疾病的发生和发展中发挥重要作用。例如，一些研究发现GPX1与心血管疾病、癌症和糖尿病等疾病的风险存在相关性。此外，一些研究还发现GPX1在免疫调节、神经保护和干细胞分化等方面发挥着重要作用。
  • 功能：
    Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism.
  • 基因功能参考文献：
    1. Knockdown of nNOS or GPx1 was specific and had broad effects on downstream genes, though it only modestly ameliorated traumatic brain injury-induced working memory deficits, despite widespread genomic changes. PMID: 29016640
    2. The glutathione peroxidase activity and p110-PI3K/Thr308-Akt are both modulated in the same way by oxygen. PMID: 24401635
    3. VPO1 plays an important role in senescence of endothelial cells and endothelial dysfunction by induction of oxidative stress and inflammatory reaction in type 2 diabetic rats. PMID: 26142204
    4. glutathione peroxidase 1 immunoreactivity was diffusely cytoplasmic in some of the hepatocytes PMID: 24867493
    5. Dietary Se-enriched A. bisporus protected against increase in ileum permeability and up-regulated GPx-1 and GPx-2 expression, selenoenzymes relevant to mitigating oxidative stress. PMID: 24962481
    6. It represents the pivotal antioxidant enzyme in vascular endothelium and exerts a protective effect against the presence of coronary artery disease PMID: 24389817
    7. data collectively indicate that GDNF is capable of inducing anti-oxidant activities of intra-cellular GPX-1 and that growth-promoting potential of GDNF and anti-oxidant properties of GPX-1 can, in concert, maximize survival of dopaminergic neurons. PMID: 23472740
    8. Upon selenium fluctuation, the modulation of UGA recoding efficiency depends on the nature of the SECIS, with Gpx1 being more sensitive than Gpx4. PMID: 22614831
    9. GPx levels in the cortex and hippocampus were increased in OLETF rats compared with LETO rats, with an inverse correlation between GPx in the hippocampus and learning score. PMID: 22981416
    10. The glutathione peroxidase represents an important part of the antioxidant defense system. PMID: 20524817
    11. expression of glutathione peroxidase 1 and glutathione reductase via GSH did not appear to coordinate with development, tissue localization or under oxidative stress PMID: 12516874
    12. excess amounts of PLOOH are efficiently eliminated through the hydrolysis by PLA2, and the subsequent reduction of FA hydroperoxide by GPx is the critical step for complete detoxification of oxidized phospholipids in the stomach PMID: 12934674
    13. Over-expression gpx1 genes in primary hippocampal or cortical cultures resulted in increased enzymatic activity of the cognate protein. PMID: 14713308
    14. Gxp1 levels was increased indicating that a reduction in oxidative damage protection may be responsible for accelerated telomere shortening over time, resulting in increased cellular senescence, loss of renal function, and death in male rats. PMID: 16189290
    15. Novel sites of Gpx1 expression were observed in colon lymphatic tissue. PMID: 16229841
    16. Our results suggest that impairment of the GPx (including glutathione)-mediated antioxidant system contributed to epileptogenesis in GEPR-9s. PMID: 18226427
    17. docosahexaenoic acid sensitizes breast cancer cells to anthracyclines through loss of glutathione peroxidase (GPx1) response PMID: 18267129
    18. Selenium requirements are higher for glutathione peroxidase-1 mRNA than gpx1 activity in rat testis. PMID: 19234057
    19. red blood cell glutathione peroxidase (Gpx1) activity in old female rats. PMID: 19398061
    20. Whole blood selenoprotein mRNA levels can be used as molecular biomarkers for assessing Selenium requirements. PMID: 19855070

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Glutathione peroxidase family
  • 组织特异性：
    Expressed in liver and lung.
  • 数据库链接：

    KEGG: rno:24404

    STRING: 10116.ENSRNOP00000066577

    UniGene: Rn.11323

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