Rat Immunoglobulin M,IgM ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠免疫球蛋白M(IgM)酶联免疫试剂盒（CSB-E07978r）为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgM含量。IgM是人体免疫系统中重要的免疫球蛋白，在早期免疫防御里发挥关键作用。其在自身免疫病、感染性疾病等的发生发展中意义重大。研究机制方面，聚焦其与抗原结合、激活补体系统等特性，探索以其为靶点开发诊断方法和治疗药物。试剂盒检测范围为6.25 ng/mL-1600 ng/mL，适用于科研领域对大鼠体液免疫状态的评估，例如感染性疾病模型中病原体特异性抗体的动态监测、疫苗研发过程中免疫原性分析，或自身免疫疾病研究中的抗体水平追踪。适用于基础免疫学、病理机制研究及生物制剂开发等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

IgM
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

6.25 ng/mL-1600 ng/mL
灵敏度：

6.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	99
	Range %	95-104
1:2000	Average %	90
	Range %	86-95
1:4000	Average %	89
	Range %	83-93
1:8000	Average %	100
	Range %	95-105

回收率：

The recovery of rat IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	92-97
EDTA plasma (n=4)	93	89-97