Rat Interleukin 1β,IL-1β ELISA Kit
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中文名称：大鼠白介素1β (IL-1β)酶联免疫试剂盒
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货号：CSB-E08055r-IS
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规格：96T/48T
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价格：￥3200/￥2500
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其他：
产品详情
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产品描述：大鼠白介素1β (IL-1β)酶联免疫试剂盒（CSB-E08055r-IS）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1B含量。IL1B 是重要的细胞因子。在炎症反应等生理病理过程中发挥关键作用。研究发现它可激活下游炎症信号通路，诱导多种炎症介质产生。针对 IL1B 研发药物，干预其作用机制，有望为炎症相关疾病如类风湿关节炎等治疗提供新策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为15.6 pg/mL。常用于评估感染性疾病、自身免疫病及神经退行性病变等研究模型；广泛用于炎症机制研究、药物抗炎效果评价、免疫调控实验及动物疾病模型构建，特别适用于脑、肝脏等组织匀浆中细胞因子释放的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：Il1bInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
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缩写：
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Uniprot No.：
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, tissue homogenates
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检测范围：31.25 pg/mL-2000 pg/mL
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灵敏度：15.6 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
91
Range %
91-105
1:2
Average %
102
Range %
95-109
1:4
Average %
99
Range %
92-105
1:8
Average %
93
Range %
86-98
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回收率：
The recovery of rat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
92
85-98
EDTA plasma (n=4)
97
90-100
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
2000
2.826
2.634
2.730
2.569
1000
2.045
1.967
2.006
1.845
500
1.349
1.328
1.339
1.177
250
0.818
0.829
0.824
0.662
125
0.474
0.491
0.482
0.321
62.5
0.344
0.353
0.349
0.187
31.25
0.238
0.244
0.241
0.080
0
0.157
0.165
0.161
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数据处理：
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货期：3-5 working days
产品评价
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靶点详情
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功能：Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
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亚细胞定位：Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
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蛋白家族：IL-1 family
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数据库链接：
STRING: 10116.ENSRNOP00000006308
UniGene: Rn.9869
