Rat Interleukin 12,IL-12/P70 ELISA KIT

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠白介素12(IL-12/P70)酶联免疫试剂盒（CSB-E07364r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL12/P70含量。IL12/P70是重要细胞因子，在免疫调节中发挥关键作用。它能激活T细胞和NK细胞来增强免疫应答。研究围绕其信号通路、与疾病关联机制展开，旨在通过调节它的活性开发治疗感染、肿瘤及自身免疫疾病的新疗法。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品适用于科研场景中免疫机制研究，如炎症性疾病模型评估、疫苗免疫效果分析或药物干预后IL - 12/P70的动态变化观测，其中血清/血浆样本可用于系统性免疫状态分析，组织匀浆则适合局部炎症微环境研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/A
缩写：

IL-12/P70
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-106
1:2	Average %	94
	Range %	91-98
1:4	Average %	93
	Range %	89-96
1:8	Average %	87
	Range %	82-96

回收率：

The recovery of rat IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	103	98-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.