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  4. Rat Interleukin 33(IL-33) ELISA Kit

Rat Interleukin 33(IL-33) ELISA Kit

文献引用 (5) 说明书下载
  • 中文名称：
    大鼠白介素33(IL-33)酶联免疫试剂盒
  • 货号：
    CSB-E14077r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠白介素33(IL-33)酶联免疫试剂盒（CSB-E14077r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL33含量。IL-33是一种前炎症细胞因子，属于IL-1家族，主要在细胞损伤时释放，诱导Th2型炎症反应。其与ST2受体结合后，激活下游信号通路，调节多种免疫细胞功能。IL-33在肿瘤、自身免疫疾病和过敏性疾病中发挥重要作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中炎症机制研究、免疫调控模型分析以及药物干预效果评估等方向，例如在呼吸道炎症模型、自身免疫疾病或创伤修复实验中定量分析IL - 33的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Il33Interleukin-33 ELISA kit; IL-33 ELISA kit
  • 缩写：
    IL33
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 103  
    Range % 98-108  
    1:2 Average % 96  
    Range % 91-101  
    1:4 Average % 89  
    Range % 83-95  
    1:8 Average % 98  
    Range % 93-104  
  • 回收率：
    The recovery of rat IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 85-94  
    EDTA plasma (n=4) 94 90-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.981 2.998 2.990 2.842  
    500 1.967 1.873 1.920 1.772  
    250 1.333 1.427 1.380 1.232  
    125 0.845 0.835 0.840 0.692  
    62.5 0.565 0.559 0.562 0.414  
    31.2 0.331 0.348 0.340 0.192  
    15.6 0.225 0.236 0.231 0.083  
    0 0.143 0.153 0.148    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

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Il33 Proteins

靶点详情

  • 功能：
    Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.; In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
  • 基因功能参考文献：
    1. The release of IL-33 from necrotic cells might induce autophagy, which can further balance the effects of increased apoptosis secondary to contrast-induced nephropathy in diabetic kidney disease. PMID: 27055881
    2. TNF-alpha stimulated IL-33/sST2 and subsequent increase of IL-6 may be the mechanism of IL-33 in acute pancreatitis. PMID: 26927343
    3. poly-IC and IL-33 play a role in myelin repair by enhancing expression of myelin genes. PMID: 27022724
    4. IL-33 protects against myocardial I/R injury by inhibiting inflammatory responses and myocardial apoptosis. PMID: 26571038
    5. These findings indicate that IL-33 might play an important role in the inflammatory response following subarachnoid hemorrhage. PMID: 25417195
    6. MRAs play a pivotal role in the Gal-3 and IL-33/ST2 modulation in post-MI cardiac remodeling. PMID: 25458175
    7. Induced interleukin-33 expression enhances the tumorigenic activity of rat glioma cells. PMID: 24327583
    8. Data indicate IL-33 concentration was increased in the pancreas in duct ligation-induced acute pancreatitis. PMID: 23418608
    9. The effects of external application of IL1beta and IL33 on the cardiomyocyte transcriptome, were compared. PMID: 23219998
    10. This study examined the expression of IL-33 and the IL-33-mediated regulation of cell functions in pancreatic stellate cells isolated from human and rat pancreas tissues. PMID: 20689058

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  • 亚细胞定位：
    Nucleus. Chromosome. Cytoplasm. Cytoplasmic vesicle, secretory vesicle. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：

    KEGG: rno:361749

    UniGene: Rn.106849

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