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中文名称：大鼠白介素4(IL-4)酶联免疫试剂盒
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货号：CSB-E04635r
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规格：96T/48T
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价格：￥3200/￥2500
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其他：
产品详情
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产品描述：CUSABIO大鼠白介素4（IL-4）酶联免疫检测试剂盒（货号：CSB-E04635r），采用双抗体夹心法定量检测大鼠血清、血浆及组织匀浆样本中的IL-4蛋白含量。IL-4作为Th2细胞分泌的关键细胞因子，在免疫调节中发挥重要作用，包括促进B细胞增殖分化、调控过敏反应及抗寄生虫免疫应答。本试剂盒检测灵敏度高，线性范围为15.6-1000 pg/mL，可精准检测低浓度样本，适用于科研领域中对炎症性疾病模型、免疫调控机制或药物干预效果的评估。实验操作基于预包被特异性抗体的96孔板，通过标准品与样本中IL-4与检测抗体的特异性结合，经显色反应实现目标蛋白的定量分析。该试剂盒兼容多种生物样本类型，为研究者探索免疫相关疾病的分子机制、疫苗开发中的Th2型免疫应答分析，以及环境因素对免疫系统影响的实验研究提供可靠工具，所有检测结果仅用于科学研究目的。
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别名：Il4 ELISA Kit; Il-4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell IGG differentiation factor ELISA Kit; B-cell growth factor 1 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
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缩写：
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Uniprot No.：
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, tissue homogenates
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检测范围：15.6 pg/mL-1000 pg/mL
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灵敏度：3.9 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
101
Range %
95-110
1:2
Average %
97
Range %
91-105
1:4
Average %
99
Range %
94-104
1:8
Average %
93
Range %
89-98
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回收率：
The recovery of human LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
90-101
EDTA plasma (n=4)
98
94-101
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
1000
3.065
2.834
2.949
2.794
500
2.708
2.567
2.637
2.482
250
2.135
2.028
2.082
1.926
125
1.434
1.429
1.431
1.276
62.5
0.774
0.721
0.748
0.592
31.2
0.468
0.453
0.461
0.305
15.6
0.307
0.294
0.300
0.145
0
0.156
0.155
0.155
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数据处理：
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货期：3-5 working days
引用文献
- Timely delivery of bone marrow mesenchymal stem cells based on the inflammatory pattern of bone injury environment to promote the repair of calvarial bone defects in rats: An optimized strategy for bone tissue engineering Y Zeng,/,2024
- Dietary Methionine Restriction Improves Gut Health and Alters the Plasma Metabolomic Profile in Rats by Modulating the Composition of the Gut Microbiota M Yang,/,2024
- Three Birds, One Stone: An Osteo-Microenvironment Stage-Regulative Scaffold for Bone Defect Repair through Modulating Early Osteo-Immunomodulation, Middle Neovascularization, and Later Osteogenesis Y Yuan,Advanced science,2023
- Stem cell biotherapy: A new remedy for Trichinella spiralis-induced inflammatory myopathy DM Abou Rayia,Parasitology international,2023
- Mesenchymal Stem Cells and Myeloid-Derived Suppressor Cells Interplay in Adjuvant-Induced Arthritis Rat Model FAM Abo-Aziza,International immunopharmacology,2023
- Taurine alleviated hepatic steatosis in oleic acid-treated-HepG2 cells and rats fed a high-fat diet Q Song,Heliyon,2023
- Tongue‐brain‐transported ZnO NPs induced abnormal taste perception A Chen,Advanced healthcare materials,2023
- Progesterone Enhances the Invasion of Trophoblast Cells by Activating PI3K/AKT Signaling Pathway to Prevent Preeclampsia H Liu,Cell transplantation,2023
- Xiaoqinglong Decoction Enhances Autophagy to Antagonist Airway Inflammation Induced by Cold in Asthmatic Rats B Wang,Evidence-based complementary and alternative medicine,2022
- In Vivo Immune Study of Achillea fragrantissima Extract Versus Echinaid and Endoxan in Wistar Rats RM Alhomaid,Current Research in Nutrition and Food Science Journal,2022
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靶点详情
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最新研究进展：IL-4是一种细胞因子，它在机体免疫反应中发挥重要作用，特别是在过敏和响应寄生虫感染时。近年来的研究表明，IL-4在多种疾病的发生和发展中也具有潜在的作用。例如，在肿瘤中，IL-4能够刺激免疫细胞对肿瘤细胞的杀伤作用，因此被视为一种潜在的肿瘤免疫治疗药物。此外，IL-4还被发现在许多自身免疫性疾病中表达水平升高，如类风湿关节炎、多发性硬化症等，因此也成为这些疾病治疗的研究热点之一。
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功能：Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
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基因功能参考文献：
- age-related decrease in IL-4 expression exacerbated surgery-induced cognitive deficits and exaggerated the neuroinflammatory responses PMID: 28414034
- IL-4 Release from a Biomimetic Scaffold for the Temporally Controlled Modulation of Macrophage Response PMID: 26951461
- Formoterol synergizes with des-ciclesonide to inhibit IL-4 expression in IgE/antigen-induced mast cells by inhibiting JNK activation. PMID: 26003274
- CaSR activation promoted T-cell apoptosis and the secretion of pro-inflammatory cytokine TNF-alpha and anti-inflammatory cytokines IL-4. PMID: 25256599
- IL-4 was found to augment the mRNA expression of ICAM-1. PMID: 23904363
- IL-4 has a modulatory effect on the enzymatic compartment of the antioxidant defense system in the brain of rats. PMID: 24319732
- IL-4 promoted the activation of effector cells to mediate rejection and did not support alloantigen-specific Treg that could transfer specific tolerance. PMID: 24139939
- The level of IL-4 is elevated in masseter muscle of both sexes following chemically induced inflammation. PMID: 20969918
- Treatment with intranasal interferon gamma can inhibit production of IL4 and IL5 in allergic rhinitis. PMID: 18510219
- IL4 in bronchoalveolar lavage fluid of allergic rhinitis rats was elevated. PMID: 16229173
- endogenous expression of antiinflammatory cytokine IL-4 regulates the extent of acute macrophage activation and confines the ensuing secondary cavity formation after spinal cord trauma PMID: 20623539
- passive rats exposed to emotional stress were characterized by a decreased blood levels of TNF alpha and IL-4 as compared to behaviorally passive controls and behaviorally active animals exposed to stress. PMID: 20120734
- A single treatment with IL-4 via retrovirally transduced lymphocytes partially protects against type 1 diabetes in BioBreeding rats. PMID: 12004164
- Gene transfer of IL-4 may represent a potential therapeutic approach to induce tolerance to splenic allografts. PMID: 15251393
- IL-4 appears to be an important Th2 cytokine involved in the induction of the L-arginine/iNOS pathway in eosinophils PMID: 15826818
- synthesis and release from mast cells mediated by Mycoplasma pneumoniae P1 adhesin PMID: 16169702
- HSV-mediated expression of IL-4 effectively reduces the behavioral manifestations of neuropathic pain, and reverses some of the biochemical and histologic correlates of neuropathic pain at the spinal level. PMID: 16503976
- Data show that islets of non-diabetic BB rats become positive to both IL-1beta and IL-4 very early on, and that production of these interleukins together with the expression of CD4 and CD25 are predictive for type 1 diabetes onset in non-diabetic BB rats. PMID: 16972262
- These data suggest that IL-4 may provide a significant immunomodulatory signal which can protect against microglia-mediated neurotoxicity by suppressing the production and release of free radicals. PMID: 17018025
- We conclude that IL-4 exerts its anti-inflammatory effects in a limited and target-specific manner, which is delicately regulated by the cellular microenvironment. T PMID: 17442043
- Our data demonstrate, for the first time, the neuroprotective effect of IL-4 through cAMP/PKA pathway in thapsigargin-induced photoreceptor cell death. PMID: 19235892
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亚细胞定位：Secreted.
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蛋白家族：IL-4/IL-13 family
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数据库链接：
