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  4. Rat Keratin, type I cytoskeletal 18(KRT18) ELISA kit

Rat Keratin, type I cytoskeletal 18(KRT18) ELISA kit

说明书下载
  • 中文名称：
    大鼠细胞角蛋白18-M65（CK 18-M65）酶联免疫试剂盒
  • 货号：
    CSB-EQ027319RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠细胞角蛋白18-M65（CK 18-M65）酶联免疫试剂盒（CSB-EQ027319RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的KRT18含量。KRT18 是角蛋白家族成员。在细胞中维持细胞结构稳定、参与信号传导。其异常表达与肿瘤等疾病密切相关。研究发现它可作为肿瘤诊断标志物，还参与肿瘤细胞增殖、迁移等过程，有望成为潜在治疗靶点。试剂盒检测范围为0.94 ng/mL-60 ng/mL，适用于科研领域中对大鼠模型的组织病理机制研究，例如肝纤维化、肝癌模型构建或化学诱导毒性实验中细胞死亡动态监测，为评估上皮细胞损伤程度及疾病进展提供定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Krt18 ELISA Kit; Krt1-18Keratin ELISA Kit; type I cytoskeletal 18 ELISA Kit; Cytokeratin-18 ELISA Kit; CK-18 ELISA Kit; Keratin-18 ELISA Kit; K18 ELISA Kit
  • 缩写：
    KRT18
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma,tissue homogenates
  • 检测范围：
    0.94 ng/mL-60 ng/mL
  • 灵敏度：
    0.23 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat KRT18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    96

    Range %

    92-100

    1:2

    Average %

    99

    Range %

    94-103

    1:4

    Average %

    101

    Range %

    98-105

    1:8

    Average %

    98

    Range %

    93-104

  • 回收率：

    The recovery of rat KRT18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    89

    84-95

    EDTA plasma (n=4)

    100

    95-105

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    60

    2.606

    2.657

    2.632

    2.469

    30

    1.798

    1.774

    1.786

    1.623

    15

    1.152

    1.198

    1.175

    1.012

    7.5

    0.713

    0.739

    0.726

    0.563

    3.75

    0.446

    0.468

    0.457

    0.294

    1.87

    0.334

    0.348

    0.341

    0.178

    0.94

    0.265

    0.267

    0.266

    0.103

    0

    0.161

    0.165

    0.163

    　

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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Krt18 Proteins

相关问答

■ 常见问题解答
Q:

1. Which is this standard in this kit, natural material or recombinant? If recombinant, please inform me on the expression method (E.coli etc.).
2. Does this kit include polyclonal antibody (except for microplate binding protein)? If so, please tell me the derivation and purification method.
3. Is the standard or antibody, including any additive agent (antiseptic agent or preserving agent)? If so, please let me know the composition and concentration.
4. Does this product include a Stop solution? If so, please tell me the composition and concentration for the stop solution.
5. Please let me know whether this kit include any hazardous substances (azide, thimerosal, 2-ME, etc). If so, please inform me of the composition and concentration.

A:
Thanks for your inquiry!
Pls check our answer:
1, Standard of CSB-EQ027319RA is NS0-derived recombinant protein
2, Coated anti: Mouse monoclonal antibody(precoated in the plate); Detection anti: Goat polyclonal(affinity purification)
3, Stop solution: 4N sulfuric acid
4, There is no hazardous substances (azide, thimerosal, 2-ME, etc) included in the kit.
5, Preservative of this kit is proclin 300(1:3000).

靶点详情

  • 功能：
    When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells.
  • 基因功能参考文献：
    1. Resutls show that the appearance caspase-cleaved CK18 in plasma was found to reflect formation of the caspase-cleaved epitope in PMID: 19407366
  • 亚细胞定位：
    Nucleus matrix. Nucleus, nucleolus. Cytoplasm.
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine
  • 数据库链接：

    KEGG: rno:294853

    STRING: 10116.ENSRNOP00000067234

    UniGene: Rn.103924

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