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  4. Rat Keratinocyte Growth Factor,KGF ELISA Kit

Rat Keratinocyte Growth Factor,KGF ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    大鼠角化细胞生长因子(KGF)酶联免疫试剂盒
  • 货号：
    CSB-E12905r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠角化细胞生长因子（KGF）的定量检测试剂盒（货号：CSB-E12905r），采用双抗体夹心ELISA技术，可灵敏检测血清、血浆样本中KGF的表达水平，检测范围覆盖31.25 pg/mL至2000 pg/mL。KGF作为成纤维细胞生长因子家族成员，在皮肤、肠道等上皮组织的增殖修复中发挥关键作用，其水平变化与组织再生、黏膜屏障功能调控等生理病理过程密切相关。本试剂盒基于高特异性抗体对，通过预包被捕获抗体和标记检测抗体的协同作用，实现对目标蛋白的精准定量分析，适用于皮肤损伤修复机制、上皮细胞再生调控、药物干预效果评估等基础研究领域，为探索KGF在组织稳态维持及疾病模型中的作用提供可靠工具。该产品操作简便，支持标准曲线定量分析，满足科研实验中对低丰度生物标志物的检测需求。
  • 别名：
    Fgf7 ELISA Kit; Fgf-7 ELISA Kit; KgfFibroblast growth factor 7 ELISA Kit; FGF-7 ELISA Kit; Heparin-binding growth factor 7 ELISA Kit; HBGF-7 ELISA Kit; Keratinocyte growth factor ELISA Kit
  • 缩写：
    FGF7
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %81-95
    1:2Average %95
    Range %89-101
    1:4Average %92
    Range %87-96
    1:8Average %86
    Range %80-93
  • 回收率：
    The recovery of rat KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10094-105
    EDTA plasma (n=4)9589-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.730 2.775 2.753 2.621
    10002.226 2.403 2.315 2.183
    5001.500 1.610 1.555 1.423
    2500.837 0.890 0.864 0.732
    1250.428 0.432 0.430 0.298
    62.50.221 0.235 0.228 0.096
    31.20.167 0.158 0.163 0.031
    00.130 0.133 0.132 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Fgf7 Proteins

靶点详情

  • 功能：
    Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
  • 基因功能参考文献：
    1. The findings of the present study indicated that KGF, CTGF and TGF-beta have biological roles in progression of CsA- and PNT- induced GO. KGF plays a greater role in CsA- induced GO than in PNT- induced GO. Meanwhile, CTGF and TGF-beta play a role in PNT- induced GO greater than in CsA- induced GO. PMID: 26894526
    2. These data demonstrate that administration of MSCs to the inflammation-insulted AT-II cells may ameliorate the impairments through a KGF-dependent PI3K/AKT/mTOR signaling pathway. PMID: 27035760
    3. The primary role of both KGF1 and FGF2 appeared to be in the proliferation and migration of keratinocytes PMID: 21136143
    4. KGF may have a role in ameliorating radiation-induced pulmonary injury in rats PMID: 21436609
    5. KGF promotes preadipocyte proliferation by an autocrine mechanism that involves activation of the PI3K/Akt signaling pathway PMID: 20069574
    6. Keratinocyte growth factor may promote alveolar epithelial repair through several mechanisms involved in cell migration PMID: 12581741
    7. Decreased gene expression of FGF-7 in the rat hypoplastic lung suggests that pulmonary hypoplasia in nitrofen-induced congenital diaphragmatic hernia rat may be caused by reduced synthesis of FGF-7 during lung morphogenesis. PMID: 12861530
    8. KGF stimulates lipogenesis in alveolar type II cells by a coordinated expression of lipogenic enzymes and transport proteins regulated by C/EBP isoforms and SREBP-1c PMID: 12865412
    9. stimulates migration and hyaluronan synthesis in the epidermis by activation of keratinocyte hyaluronan synthases 2 and 3 PMID: 14506240
    10. Exogenous administered KGF cDNA causes increases in IGF-I, IGF-BP3, FGF, and collagen IV and decreases TGF-beta concentration. KGF gene transfer accelerates wound healing without causing an increase in collagen I or III. PMID: 17292422
    11. KGF improves alterations of lung permeability and bronchial epithelium in allergic rats PMID: 17392324
    12. retention of the AT2 cell phenotype by KGF involves c-Jun and suggest that activation of c-Jun kinase may be an important determinant of maintenance of AT2 cell phenotype PMID: 17872496
    13. FGF-7, most likely through its effect on the vascular bed, is required for normal early postnatal alveolar formation from secondary crests. PMID: 18091341
    14. the effects of KGF on duct cell proliferation are mediated by the MEK-ERK1/2 pathway, while the KGF-induced cell differentiation is mediated by the PI3K/AKT pathway PMID: 19266047

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  • 蛋白家族：
    Heparin-binding growth factors family
  • 数据库链接：

    KEGG: rno:29348

    STRING: 10116.ENSRNOP00000012700

    UniGene: Rn.98842

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