Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠NAD依赖性蛋白脱乙酰酶sirtuin-1(SIRT1)酶联免疫试剂盒（CSB-EL021339RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SIRT1含量。SIRT1 是一种具有重要生物学意义的靶点。它参与细胞代谢、衰老、应激反应等多种生理过程。研究发现它通过去乙酰化作用调控下游蛋白功能，在癌症、代谢疾病等方面有潜在机制，是当前药物研发和基础研究关注的重要靶点。试剂盒检测范围为34.3 pg/mL-2200 pg/mL，适用于体外研究，如通过检测不同处理条件下（如药物干预、基因修饰）的SIRT1表达变化，探索其在能量稳态调节、神经保护或抗氧化通路中的作用机制，也可用于肥胖、糖尿病等代谢模型动物的生物标志物分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

RP11-57G10.3, SIR2L1, SIR2alpha|sir2-like 1|sirtuin 1|sirtuin type 1
缩写：

SIRT1
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

34.3 pg/mL-2200 pg/mL
灵敏度：

8.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SIRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-94
1:2	Average %	102
	Range %	97-105
1:4	Average %	91
	Range %	87-95
1:8	Average %	98
	Range %	92-102

回收率：

The recovery of rat SIRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	99	95-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.