Rat Neuron-specific enolase,NSE ELISA Kit
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中文名称：
大鼠神经特异性烯醇化酶(NSE)酶联免疫试剂盒
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货号：
CSB-E07963r
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规格：
96T/48T
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价格：
￥3900/￥2500
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其他：
产品详情
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产品描述：
大鼠神经特异性烯醇化酶(NSE)酶联免疫试剂盒（CSB-E07963r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ENO2含量。ENO2即烯醇化酶 2，在神经组织等中高表达。它参与糖酵解过程，为细胞提供能量。异常表达与神经母细胞瘤等肿瘤发生发展相关，可作为肿瘤诊断标志物与治疗靶点，研究其机制有助于开发针对性治疗策略。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于实验动物模型（如脑损伤、神经退行性疾病）中NSE的动态监测，或体外培养神经细胞的功能研究，可支持多种样本类型的批量检测，为探索神经系统疾病机制、评估神经损伤程度或药物干预效果提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：
Eno2 ELISA Kit; Eno-2 ELISA Kit; Gamma-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 2 ELISA Kit; Neural enolase ELISA Kit; Neuron-specific enolase ELISA Kit; NSE ELISA Kit
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缩写：
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Uniprot No.：
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种属：
Rattus norvegicus (Rat)
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样本类型：
serum, plasma, tissue homogenates
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检测范围：
0.625 ng/mL-40 ng/mL
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灵敏度：
0.156 ng/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Metabolism
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 85-94 | |
| 1:2 | Average % | 100 | |
| Range % | 97-104 | |
| 1:4 | Average % | 90 | |
| Range % | 87-94 | |
| 1:8 | Average % | 100 | |
| Range % | 95-103 | |
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回收率：
| The recovery of rat NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 91-98 | |
| EDTA plasma (n=4) | 95 | 92-97 | |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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 | ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.260 | 2.232 | 2.246 | 2.078 | | | 20 | 1.454 | 1.486 | 1.470 | 1.302 | | | 10 | 0.920 | 0.928 | 0.924 | 0.756 | | | 5 | 0.654 | 0.663 | 0.659 | 0.491 | | | 2.5 | 0.403 | 0.398 | 0.401 | 0.233 | | | 1.25 | 0.322 | 0.314 | 0.318 | 0.150 | | | 0.625 | 0.266 | 0.257 | 0.262 | 0.094 | | | 0 | 0.170 | 0.166 | 0.168 | | |
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数据处理：
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货期：
3-5 working days
引用文献
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Glycyrrhiza uralensis Fisch. and its active components mitigate Semen Strychni-induced neurotoxicity through regulating high mobility group box 1 (HMGB1) translocation
X Duan,Biomedicine & pharmacotherapy,2023
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Reversing Postcardiopulmonary Bypass Associated Cognitive Dysfunction Using k-Opioid Receptor Agonists to Regulate Microglial Polarization via the NLRP3/Caspase-1 Pathway
Pei Song,Journal of Healthcare Engineering,2021
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Dexmedetomidine reduces the neuronal apoptosis related to cardiopulmonary bypass by inhibiting activation of the JAK2?STAT3 pathway
Chen Y.et al,Drug Des Devel Ther,2017
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Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis
Miaoyun Wen.et al,Experimental
and Therapeutic
Medicine,2017
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Hypothermia followed by rapid rewarming exacerbates ischemia-induced brain injury and augments inflammatory response in rats.
Zhu SZ.et al,Biochem Biophys Res Commun.,2016
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Endotoxin-induced lung alveolar cell injury causes brain cell damage
Rodr铆guez-Gonz谩lez R.et al,Exp Biol Med (Maywood).,2015
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Understanding the anti-kindling role and its mechanism of Resveratrol in Pentylenetetrazole induced-kindling in a rat model
Saha L et al,Pharmacol Biochem Behav.,2014
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Therapeutic time window of hypothermia is broader than cerebral artery flushing in carotid saline infusion after transient focal ischemic stroke in rats
/,Neurological Research,,2012
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Interrupted intracarotid artery cold saline infusion as an alternative method for neuroprotection after ischemic stroke
Ya-Bin Ji et al,Neurosurgical Focus,2012
靶点详情
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功能：
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
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亚细胞定位：
Cytoplasm. Cell membrane.
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蛋白家族：
Enolase family
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组织特异性：
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
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数据库链接：
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