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中文名称:大鼠神经营养因子3(NT-3)酶联免疫试剂盒
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货号:CSB-E04688r
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规格:96T/48T
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价格:¥3900/¥2500
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其他:
产品详情
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产品描述:CUSABIO大鼠神经营养因子3(NT-3)酶联免疫检测试剂盒(货号CSB-E04688r),采用双抗体夹心法原理实现定量分析,可灵敏检测大鼠血清、血浆及组织匀浆样本中NT-3蛋白的表达水平。NT-3是神经营养因子家族重要成员,在神经元存活、突触可塑性及神经损伤修复中发挥关键作用,其动态变化与神经系统发育和功能调控密切相关。试剂盒检测范围为0.9-60 ng/mL,最低检测限达0.9 ng/mL,具备高特异性和重复性,预包被板及优化试剂组分可确保实验流程简便高效,适用于神经退行性疾病机制研究、神经再生模型评估或药物干预效果分析等科研场景。操作时仅需100μL样本量,通过标准曲线计算目标浓度,配套提供试剂盒组分包括预包被微孔板、检测抗体、标准品、显色液及终止液等,实验全程耗时约4.5小时,需配备酶标仪(450nm波长)完成吸光度测定。该试剂盒为探索NT-3在神经生物学中的功能及调控网络提供了可靠的科研工具。
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别名:Ntf3 ELISA Kit; Ntf-3 ELISA Kit; Neurotrophin-3 ELISA Kit; NT-3 ELISA Kit; HDNF ELISA Kit; Nerve growth factor 2 ELISA Kit; NGF-2 ELISA Kit; Neurotrophic factor ELISA Kit
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缩写:
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Uniprot No.:
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, tissue homogenates
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检测范围:0.9 ng/mL-60 ng/mL
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灵敏度:0.225 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Neuroscience
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 88-96 1:2 Average % 94 Range % 89-98 1:4 Average % 97 Range % 93-101 1:8 Average % 95 Range % 89-98 -
回收率:
The recovery of rat NT-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 88 82-94 EDTA plasma (n=4) 92 86-98 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 60 2.512 2.619 2.566 2.434 30 2.235 2.121 2.178 2.046 15 1.526 1.504 1.515 1.383 7.5 0.812 0.823 0.818 0.686 3.75 0.458 0.446 0.452 0.320 1.8 0.326 0.314 0.320 0.188 0.9 0.221 0.227 0.224 0.092 0 0.133 0.131 0.132 -
数据处理:
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货期:3-5 working days
引用文献
- Low-intensity pulsed ultrasound promotes proliferation and differentiation of neural stem cells to enhance spinal cord injury recovery YH Liao, MH Tian, WY Zhou, BQ He, C Tang,Molecular Biology Reports,2025
- Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain WJ Song,Toxics,2023
- Histological and functional outcomes in a rat model of hemisected spinal cord with sustained VEGF/NT-3 release from tissue-engineered grafts ZX Xu,Artif Cells Nanomed Biotechnol,2019
- Alcohol-induced cognitive deficits are associated with decreased circulating levels of the neurotrophin BDNF in humans and rats Daniel Silva鈥怭ena.et al,Addiction Biology,2018
- Enteric glial cell activation protects enteric neurons from damage due to diabetes in part via the promotion of neurotrophic factor release P. Luo.et al,NEUROGASTROENT MOTIL,2018
相关产品
靶点详情
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功能:Seems to promote the survival of visceral and proprioceptive sensory neurons.
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基因功能参考文献:
- the combined delivery of NT-3 and anti-NogoA from the DDS in an impact/compression model of SCI increases axon density and improves locomotor function. PMID: 27056081
- Compared to the sham group, the expression levels of NT-3 were significantly decreased and electroacupuncture was shown to upregulate the expression of NT-3 PMID: 27597902
- results demonstrate that BDNF and NT3 regulate axon initial segmen location and neuronal excitability PMID: 28440877
- Neurotrophin 3 activity in spinal cord was higher in balance and coordination trained animals following sciatic nerve crush compared to endurance trained. PMID: 26316168
- The expressed BDNF and NT3 exert a synergistic effect, which may promote the neuronal differentiation of adiposederived stem cells. PMID: 26239042
- These results indicate the feasibility of fabricating NT-3-immobilized scaffolds using the adsorption of NT-3/SF coating method, as well as the potential of these scaffolds to induce SCI repair by promoting survival and neuronal differentiation PMID: 25215612
- NT-3 gene-modified bone marrow-derived mesenchymal stem cells via lumbar puncture can strengthen the therapeutic benefits of bone mesenchymal stem cell transplantation PMID: 24744011
- A method of hypoglossal-facial nerve anastomosis through an NT3-expressing predegenerated nerve graft engineered by lentiviral vectors is designed to treat complete facial palsy in adult rats. PMID: 23581595
- Our results show that NT-3 modifies the ability of the mossy fiber pathway to present subsequent long-term potentiation PMID: 23387385
- mRNA expression of brain-derived neurotrophic factor (BDNF) and NT-3 (but not nerve growth factor) in injury segment follows neural stem cell transplantation and is remarkably upregulated. PMID: 22573254
- The exposure to long-term stress likely affects a variety of organs capable of releasing NT-3 into the bloodstream. Elevation of plasma NT-3 levels may play important roles in homeostasis under stress conditions. PMID: 23074106
- We conclude that upregulation of NT-3 in neonatally wounded skin is a critical factor mediating the sensory nerve sprouting that underlies hypersensitivity and pain following skin injury. PMID: 22871470
- Upregulation of neurotrophin-3 in PC12 cells reduces apoptosis under hypoxic conditions. PMID: 22960790
- The NTF3 gene promoted differentiation of neural stem cells into cholinergic neurons and enhanced neuronal cell survival PMID: 22971496
- Accompanying the simulated weightlessness extension, NT-3 expression in rat soleus muscle spindles is progressively decreased. PMID: 21340438
- Transient receptor potential canonical (TRPC)5 acts as a novel and specific mediator for NT-3 to regulate dendrite development through calcium/calmodulin-dependent protein kinase (CaMK)IIalpha. PMID: 22764246
- Muscle NT-3 levels increased by exercise training contribute to the improvement in caudal nerve conduction velocity in diabetic rats. PMID: 22552353
- Endogenous NT-3 may be involved in the analgesic effect of electroacupuncture on inflammatory pain in rats, mediated through the inhibition of spinal glial activity. PMID: 21680256
- Neurotrophin 3 modulates neuropathic pain in the dorsal root ganglia in a rat model. PMID: 21221027
- MiR21 is a candidate for regulating neurotrophin-3 signaling in the hippocampus following status epilepticus. PMID: 22019057
- neurotrophin 3 signaling pathway is essential for opiate-induced adaptation of locus coeruleus neurons. PMID: 17688944
- NT-3 immunoreactivity increased in activated fibers after partial transection of facial nerve. PMID: 21473141
- Neurotrophin 3 may lead to enhanced exocytosis of synaptic vesicles. PMID: 21370927
- regular rhythms of BDNF and NT-3 are essential for correct cortical network formation in juvenile rodents PMID: 21527636
- regrowth and perception were improved when the nerve graft overexpressed neurotrophin-3 at the time of transplantation PMID: 21135740
- Endogenous NT-3 has a distinctive role in promoting spiral ganglion axon growth in the organ of Corti and synaptogenesis on inner hair cells. PMID: 21613508
- Results suggest that proneurotrophin-3 and proBDNF may play important roles in the response to noise-induced injuries or ototoxic damage via the Sortilin:p75(NTR) death-signalling complex. PMID: 21261755
- 3'UTR of calreticulin has two conserved regions, and either of these is sufficient for axonal and dendritic targeting; only nucleotides 1315-1412 show ligand responsiveness to neurotrophin 3 (NT3) and myelin-associated glycoprotein (MAG) PMID: 20308067
- Report the proangiogenic capacity of NT-3 and propose NT-3 as a novel potential agent for the treatment of ischemic disease. PMID: 20360537
- Our results indicate for the first time that monoaminergic neurotransmitters play an important role in the regulation of NT-3 synthesis in cultured rat astrocytes. PMID: 19854260
- Brain-derived neurotrophic factor (BDNF) levels in the spinal cord, as detected by enzyme-linked immunosorbent assay, decreased with time, whereas neurotrophin-3 (NT-3) levels remained unchanged. PMID: 19203225
- exercise decreases hippocampal neurotrophin-3 PMID: 12914971
- ability of neurotrophin-3 (NT-3) to prevent abnormalities in neurofilamens in sensory neurons in experimental diabetes PMID: 12941778
- neurotrophin-3 (NT-3), delivered chronically via fibroblasts implanted intrathecally into neonatal rats, can facilitate synaptic transmission in the spinal cord PMID: 14507970
- Transfection of NT-3 into oligidendrocyte precursors caused a 10-fold increase in myelin basic protein production observed in myelin membranes in cocultures with hippocampal neurons. PMID: 15139015
- the level of supraspinal and muscle afferent input plays an important role in modulating the levels of BDNF and NT-3 in the spinal cord. PMID: 15548637
- These data demonstrate that localized overexpression of BDNF or NT-3, but not NGF, is sufficient to promote sprouting of serotonergic axons in the cortex following an experimental neurotoxic injury. PMID: 16206279
- BDNF, NT-3, and NGF, secreted by transplanted neural stem cells, were involved in the promotion of corticospinal axon growth after transplantation PMID: 17515814
- Glutamate receptor blockade inhibited the generation of spontaneous sodium-driven action potentials which, in turn, regulate both the endogenous secretion of NT-3 and the calbindin-D(28k) phenotype acquisition. PMID: 17561837
- neurotrophin-3 is a unique hypertrophic factor, which is paradoxically downregulated in cardiac hypertrophy and might counteract hypertrophic change PMID: 17628607
- Biochemically, the XYS decoction also reversed CIS-induced decreases in BDNF and increases in TrkB and NT-3 in the frontal cortex and the hippocampal CA(1) subregion. PMID: 17647101
- Vascular-derived NT-3 increases or maintains neuropeptide Y and tyrosine hydroxylase expression in postganglionic sympathetic neurons PMID: 18032527
- The functional consequences of the effect of IL-1beta on NT-3 signaling were severe, as NT-3 protection of the trophic support-deprived cortical neurons was abrogated. PMID: 18036576
- Increased NT-3 mRNA expression in hippocampus was detected in the immobilization stress group; the magnitude of increase in NT-3 mRNA was decreased when animals were allowed to bite. PMID: 18191115
- Co-culture of Ntf3 over-expressing Schwann cells with SGNs from early postnatal rats significantly enhanced neuronal survival in comparison to both control Schwann cells and conventional recombinant neurotrophin proteins. PMID: 18304740
- Non-viral vector-mediated genetic engineering of OEG was safe and more effective in producing NT-3 and promoting axonal outgrowth followed by enhancing SCI recovery in rats. PMID: 18369383
- Exposure of neurons to increased levels of NT-3 reduces expression of sodium channels Na(v)1.8 and Na(v)1.9 in dorsal root ganglia neurons subject to chronic constriction injury. PMID: 18601922
- levels in cerebellar vermis were higher for animals in an enriched environment after prenatal alcohol exposure PMID: 18652597
- Analysis of neurotrophin expression in dentate gyrus dosen't reveal an expression change of Ntf3 in a genetically hypertensive strain of Wistar rat. PMID: 18957307
- findings suggest that orexins, but not melanin-concentrating hormone, may be an inducer of NT-3 in the cerebral cortex PMID: 19026718
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亚细胞定位:Secreted.
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蛋白家族:NGF-beta family
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组织特异性:Brain and peripheral tissues.
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数据库链接: