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  4. Rat Neurotrophin 4,NT-4 ELISA KIT

Rat Neurotrophin 4,NT-4 ELISA KIT

文献引用 (1) 说明书下载
  • 中文名称：
    大鼠神经营养因子4(NT-4)酶联免疫试剂盒
  • 货号：
    CSB-E04691r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO大鼠神经营养因子4（NT-4）酶联免疫检测试剂盒（货号：CSB-E04691r），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的NT-4蛋白含量。NT-4作为神经营养因子家族成员，在神经元存活、突触可塑性及神经再生等生理过程中发挥重要作用。该试剂盒检测灵敏度高，线性范围为47 pg/mL至3000 pg/mL，可精准覆盖生理及实验条件下NT-4的浓度变化。实验操作基于特异性抗体对目标蛋白的捕获与标记检测，支持科研人员通过标准曲线进行样本浓度计算。本试剂盒适用于神经发育、损伤修复机制研究，以及神经退行性疾病模型、药物干预实验中NT-4的动态监测，例如脑组织、外周神经或血液样本的分析。产品包含预包被96孔板、标准品、检测抗体及显色系统等完整组分，提供标准化操作流程，满足体外实验对NT-4定量分析的需求。
  • 别名：
    Ntf4 ELISA Kit; Nt4 ELISA Kit; Ntf5 ELISA Kit; Neurotrophin-4 ELISA Kit; NT-4 ELISA Kit; Neurotrophin-5 ELISA Kit; NT-5 ELISA Kit; Neutrophic factor 4 ELISA Kit
  • 缩写：
    NTF4
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    47 pg/mL-3000 pg/mL
  • 灵敏度：
    11.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %82-94
    1:2Average %93
    Range %88-98
    1:4Average %96
    Range %90-102
    1:8Average %99
    Range %95-104
  • 回收率：
    The recovery of rat NT-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-102
    EDTA plasma (n=4)10095-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    30002.268 2.250 2.259 2.150
    15001.845 1.871 1.858 1.749
    7501.464 1.492 1.478 1.369
    3751.135 1.149 1.142 1.033
    187.50.704 0.724 0.714 0.605
    940.438 0.457 0.448 0.339
    470.287 0.294 0.291 0.182
    00.108 0.110 0.109 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Ntf4 Proteins

靶点详情

  • 功能：
    Could serve as a target-derived trophic factor for sensory and sympathetic neurons.
  • 基因功能参考文献：
    1. The neuroprotective effect of NT-4 on retinas cultured under diabetic stress correlates with suppression of PERK kinase and transcription factor CHOP. PMID: 21767604
    2. alteration of NT-4 protein levels and gene expression in brain stem, cerebellum and cerebral hemisphere in context of ecstasy dependence; results show chronic administration of ecstasy significantly increased NT-4 gene expression in these areas of brain PMID: 21273656
    3. NT-4, alone or when co-expressed with brain-derived neurotrophic factor, is processed within and secreted by the constitutive secretory pathway PMID: 12970359
    4. The results indicate that oleic acid behaves as a neurotrophic factor, inducing GAP-43 expression through a PKC-mediated mechanism that is not mediated by other neurotrophic factors but that is strongly synergized by NT-3 and NT-4/5. PMID: 14519521
    5. NT-4 significantly improved slow motor unit recovery, and provides a basis for therapies intended to aid the functional recovery of muscles after denervating injury. PMID: 14622146
    6. BDNF and NT-4 expression could be modulated by activity of spinal circuitry and further support putative involvement of the endogenous neurotrophins in mechanisms of spinal neuroplasticity. PMID: 15193526
    7. Transcellular induction of neuropeptide Y expression by NT4 and BDNF. PMID: 15703301
    8. NT-4 regulates proliferation and differentiation of the dental epithelium and promotes production of the enamel matrix PMID: 18045880
    9. Analysis of neurotrophin expression in dentate gyrus dosen't reveal an expression change of Ntf4 in a genetically hypertensive strain of Wistar rat. PMID: 18957307

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    NGF-beta family
  • 组织特异性：
    Expressed in thymus, muscle, ovary, brain, heart, stomach and kidney. Expressed in both embryo and adult tissues.
  • 数据库链接：

    KEGG: rno:25730

    STRING: 10116.ENSRNOP00000028195

    UniGene: Rn.44225

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