Rat Pancreatic alpha-amylase(AMY2A) ELISA kit

产品详情

文献引用 (3) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO针对大鼠胰淀粉酶α2A（AMY2A）的定量检测试剂盒（货号：CSB-EL001689RA），AMY2A是胰腺外分泌功能的关键标志物，主要负责淀粉类碳水化合物的水解，其水平变化常与胰腺生理状态及代谢调控相关。试剂盒采用双抗体夹心法ELISA原理，可精准检测血清、血浆及组织匀浆样本中3.12-200 mIU/mL浓度范围的AMY2A，最低检测限达3.12 mIU/mL，具备高灵敏度和特异性。该产品适用于科研领域中对大鼠胰腺功能的基础研究，如评估营养代谢干预、胰腺炎模型或糖尿病等代谢性疾病动物模型的病理机制，也可用于药物开发中胰腺相关生物标志物的动态监测。实验操作流程标准化，提供预包被捕获抗体和生物素化检测抗体，兼容常规酶标仪进行吸光度分析，支持研究者高效获取稳定可靠的实验数据。
缩写：

AMY2A
Uniprot No.：

P00689
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 mIU/mL-200 mIU/mL
灵敏度：

0.78 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	95-104
1:2	Average %	88
	Range %	84-93
1:4	Average %	90
	Range %	85-94
1:8	Average %	99
	Range %	94-105

回收率：

The recovery of rat AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-101
EDTA plasma (n=4)	92	88-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

基因功能参考文献：
1. Comparative proteomic analysis revealed that amylase levels were decreased by twofold in pancreases from hyperlipidemic rats compared with control rats.Pancreatic amylase protein levels were also decreased in tissue from hyperlipidemic rats. PMID: 20981735
2. analysis of how amylase secretion is proteoglycan-dependent in rat parotid acinar cells PMID: 17980695
亚细胞定位：

Secreted, extracellular space.
蛋白家族：

Glycosyl hydrolase 13 family
数据库链接：

KEGG: rno:497039

STRING: 10116.ENSRNOP00000022268

UniGene: Rn.67070