Rat Podocalyxin,PCX ELISA Kit
产品详情
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产品描述：
CUSABIO大鼠足细胞标记蛋白/足盂蛋白（PCX）酶联免疫检测试剂盒（货号：CSB-E13946r），采用双抗体夹心法定量分析样本中PCX含量，适用于血清、尿液及细胞培养上清液等多种样本类型。PCX作为足细胞特异性跨膜糖蛋白，是肾小球滤过屏障的重要组成部分，其表达水平变化常与肾小球损伤、蛋白尿等病理过程相关，是研究肾脏疾病机制的重要生物标志物。本试剂盒检测灵敏度覆盖1.56 ng/mL至100 ng/mL线性范围，通过预包被高特异性抗体实现精准捕获与信号放大，操作简便且重复性良好。科研人员可通过该试剂盒动态监测实验动物或细胞模型中PCX的表达变化，用于探究肾脏疾病发生发展机制、评估药物干预效果或建立体外足细胞损伤模型等基础研究。实验仅需100 μL样本量，兼容常规酶标仪检测，为实验室提供高效、稳定的科研工具。
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别名：
Podxl ELISA Kit; Pclp1 ELISA Kit; Podocalyxin ELISA Kit; Podocalyxin-like protein 1 ELISA Kit; PC ELISA Kit; PCLP-1 ELISA Kit
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缩写：
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Uniprot No.：
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种属：
Rattus norvegicus (Rat)
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样本类型：
serum, urine, cell culture supernates
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检测范围：
1.56 ng/mL-100 ng/mL
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灵敏度：
0.39 ng/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Cancer
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PCX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 81-93 | |
| 1:2 | Average % | 97 | |
| Range % | 93-101 | |
| 1:4 | Average % | 89 | |
| Range % | 85-93 | |
| 1:8 | Average % | 98 | |
| Range % | 92-104 | |
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回收率：
| The recovery of rat PCX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 85-97 | |
| EDTA plasma (n=4) | 89 | 83-94 | |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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 | ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.201 | 2.312 | 2.257 | 2.112 | | | 50 | 1.574 | 1.456 | 1.515 | 1.370 | | | 25 | 1.009 | 1.056 | 1.033 | 0.888 | | | 12.5 | 0.633 | 0.654 | 0.644 | 0.499 | | | 6.25 | 0.401 | 0.412 | 0.407 | 0.262 | | | 3.12 | 0.272 | 0.286 | 0.279 | 0.134 | | | 1.56 | 0.210 | 0.215 | 0.213 | 0.068 | | | 0 | 0.144 | 0.146 | 0.145 | | |
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数据处理：
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货期：
3-5 working days
靶点详情
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功能：
Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
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基因功能参考文献：
- podocalyxin has a role in defining the structure of the podocyte basal surface PMID: 15226400
- Podocalyxin might be associated with the malignant progression of astrocytic tumors, and be a useful prognostic marker for astrocytic tumors. PMID: 18639524
- Report ubiquitous yet distinct expression of podocalyxin on vascular surfaces in normal and tumor tissues in the rat. PMID: 19142011
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亚细胞定位：
Apical cell membrane. Cell projection, microvillus. Membrane raft. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Membrane; Single-pass type I membrane protein.
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蛋白家族：
Podocalyxin family
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组织特异性：
Glomerular epithelium cell (podocyte) (at protein level).
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数据库链接：
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