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  4. Rat Polypeptide YY(PYY)ELISA Kit

Rat Polypeptide YY(PYY)ELISA Kit

文献引用 (3) 说明书下载
  • 中文名称：
    大鼠多肽YY/酪酪肽(PYY)酶联免疫试剂盒
  • 货号：
    CSB-E13432r
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO专为大鼠多肽YY/酪酪肽（PYY）检测设计的定量酶联免疫试剂盒（货号：CSB-E13432r），采用双抗体夹心法原理，可精准检测血清、血浆、细胞培养上清液及组织匀浆样本中的PYY含量，检测范围为7.8-500 pg/mL，最低检测灵敏度达7.8 pg/mL。PYY是一种由肠道L细胞分泌的胃肠激素，通过作用于中枢神经系统调控食欲和能量代谢，其水平变化与肥胖、糖尿病等代谢性疾病研究密切相关。本试剂盒包含预包被捕获抗体的酶标板、生物素标记检测抗体、标准品及显色试剂，操作简便且检测时间短，样本前处理仅需常规离心或匀浆步骤，试剂组分可于4℃短期保存或-20℃长期储存。适用于科研领域中对大鼠模型能量代谢机制、胃肠功能调控或肥胖相关疾病的研究，例如通过检测血清或组织中的PYY水平评估饮食干预、药物处理或基因修饰对代谢通路的影响，亦可用于体外细胞实验探究激素分泌调控机制。
  • 别名：
    PyyPeptide YY ELISA kit; PYY ELISA kit; Peptide tyrosine tyrosine) [Cleaved into: Peptide YY(3-36 ELISA kit; PYY-II)] ELISA kit
  • 缩写：
    PYY
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %90-100
    1:2Average %88
    Range %84-93
    1:4Average %90
    Range %85-94
    1:8Average %99
    Range %94-105
  • 回收率：
    The recovery of rat PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9792-101
    EDTA plasma (n=4)10095-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.478 2.374 2.426 2.318
    2501.754 1.852 1.803 1.695
    1251.188 1.226 1.207 1.099
    62.50.725 0.689 0.707 0.599
    31.20.403 0.382 0.393 0.285
    15.60.272 0.257 0.265 0.157
    7.80.189 0.178 0.184 0.076
    00.110 0.105 0.108
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Pyy Proteins

靶点详情

  • 功能：
    This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
  • 基因功能参考文献：
    1. Peptide YY mediates the satiety effects of diets enriched with whey protein fractions in male rats PMID: 29042449
    2. Additional analysis of the findings demonstrated significant interactions of ovarian cycle stage and fed/fasted status with age on GLP-1, but not PYY plasma concentrations PMID: 28237410
    3. These findings indicate that the mechanism underlying type 2 diabetes remission may be mediated by PYY. PMID: 27117413
    4. Serum PYY levels were nonsignificantly decreased in rats with human neuroblastoma and hepatoblastoma transplants. PMID: 25111274
    5. gut PYY(3-36) action at Y2 receptors peripheral to the blood brain barrier plays an essential role in mediating satiety PMID: 23482449
    6. RACK1 modulates the ability of preglomerular vascular smooth muscle cells to respond to the proliferative actions of PYY. PMID: 23303411
    7. Exercise in hypoxia suppressed hunger and increased PYY but did not influence ad libitum energy intake. PMID: 22114179
    8. PYY concentrations in plasma, descending colon and rectum tissues were increased (P < 0.001) throughout pregnancy and lactation. PYY peaked at day 5 of lactation in plasma, as well as descending colon and rectum tissues. PMID: 19778563
    9. PYY induced a contraction of smooth muscle strips from proximal colon, but not on smooth muscle from any other gut segments. PMID: 12383864
    10. findings indicate that insulin-like growth factor-I functions as a positive regulator of peptide YY gene expression and that the stimulatory effect may be mediated by Sp1 proteins PMID: 14592960
    11. PYY(3-36) might play a role in the neuroendocrine modulation of the reproductive axis PMID: 15564330
    12. observations indicate that Peptide YY (PYY) levels are influenced by age, thyroid hormones, and growth hormone and indicate that PYY could be involved in the changes of food intake associated with these conditions PMID: 15687395
    13. study indicates that peripheral Peptide YY (PYY) may transmit satiety signals to the brain in part via the vagal afferent pathway PMID: 15718279
    14. PYY-immunoreactive (ir) cell bodies were localized exclusively to the gigantocellular reticular nucleus (Gi) of the rostral medulla. The highest density of PYY fibers was present within the solitary tract nucleus. PMID: 18022952
    15. Demonstrate the development of a potent homeostatic response to increase food intake when PYY(3-36) reduces food intake and energy reserves in diet-induced obese rats. PMID: 18550871

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    NPY family
  • 数据库链接：

    KEGG: rno:287730

    STRING: 10116.ENSRNOP00000028323

    UniGene: Rn.13173

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