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  4. Rat Protein CYR61(CYR61) ELISA kit

Rat Protein CYR61(CYR61) ELISA kit

说明书下载
  • 中文名称：
    大鼠CYR61蛋白(CYR61)酶联免疫试剂盒
  • 货号：
    CSB-EL006463RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠CYR61蛋白（CYR61/Cysteine-rich angiogenic inducer 61）酶联免疫检测试剂盒（货号：CSB-EL006463RA），采用双抗体夹心法原理实现定量分析，适用于检测大鼠血清、血浆及组织匀浆样本中CYR61蛋白的表达水平。CYR61属于CCN蛋白家族成员，参与调控细胞增殖、迁移、血管生成及细胞外基质重塑等生物学过程，在组织修复、胚胎发育及病理状态如纤维化、肿瘤微环境调控中具有重要作用。该试剂盒检测范围为31.25-2000 pg/mL，灵敏度达31.25 pg/mL，可精准检测微量样本中CYR61的浓度变化。试剂盒包含预包被捕获抗体、生物素标记检测抗体、标准品及实验所需缓冲液等核心组分，操作流程标准化，适用于心血管疾病模型、肿瘤机制研究、炎症反应评估或再生医学等领域的基础科研，通过检测体液或组织样本中CYR61的动态变化，为探索其分子功能及信号通路提供可靠工具。
  • 别名：
    Ccn1 ELISA kit; Cyr61 ELISA kit; Igfbp10CCN family member 1 ELISA kit; Cellular communication network factor 1 ELISA kit; Cysteine-rich angiogenic inducer 61 ELISA kit; Insulin-like growth factor-binding protein 10 ELISA kit; IBP-10 ELISA kit; IGF-binding protein 10 ELISA kit; IGFBP-10 ELISA kit; Protein CYR61 ELISA kit
  • 缩写：
    CYR61
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CYR61 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %87-97
    1:2Average %95
    Range %91-99
    1:4Average %98
    Range %96-101
    1:8Average %99
    Range %96-104
  • 回收率：
    The recovery of rat CYR61 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9892-102
    EDTA plasma (n=4)9690-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.625 2.529 2.577 2.413
    10002.062 1.956 2.009 1.845
    5001.384 1.366 1.375 1.211
    2500.871 0.881 0.876 0.712
    1250.551 0.539 0.545 0.381
    62.50.342 0.351 0.347 0.183
    31.250.249 0.255 0.252 0.088
    00.164 0.163 0.164 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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Cyr61 Proteins

靶点详情

  • 功能：
    Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3.
  • 基因功能参考文献：
    1. Compared with wild rats, Cyr61 was overexpressed in the lung tissue, pulmonary arterial and PASMCs in PAH rats. Exogenous recombinant Cyr61 protein promoted the proliferation of PASMCs in a dose-dependent manner. While the expression of Cyr61 in PASMCs was inhibited by specific siRNA, cell proliferation was restrained and the expression of p-AKT declined PMID: 28824319
    2. the matricellular protein CCN1 gene transfer induces hepatic stellate cell apoptosis through endoplasmic reticulum stress and unfolded protein response PMID: 26515130
    3. Cyclic AMP-induced actin-cytoskeleton remodelling regulates expression of CCN1 through MKL1. PMID: 25446180
    4. These findings implicate CCN1 as a mediator of cardioprotection induced by G-protein coupled receptor agonists that activate RhoA/MRTF-A signaling. PMID: 25106095
    5. In line with dermal fibrosis and scar formation, CCN1/CYR61 is involved in liver injury repair and tissue remodeling. CCN1/CYR61 gene transfer into extracellular matrix-producing liver cells is therefore potentially beneficial in liver fibrotic therapy PMID: 24487063
    6. Muscle progenitor cells treated with aging rodent serum caused CCN1 mRNA to increase by seven fold. PMID: 24196529
    7. Report assays with site-direct mutation analysis of CYR61/CCN1 promoter demonstrated that MRTF-A transactivated CYR61/CCN1 promoter mainly depending on CArG box. PMID: 23624342
    8. Degradome products of the matricellular protein CCN1 as modulators of pathological angiogenesis in the retina. PMID: 23798676
    9. Cyr61, an angiogenic factor with previously unknown neuronal function, is a novel regulator of dendritic growth. PMID: 23362279
    10. CYR-61 is important in the pathogenesis of rheumatoid arthritis, and SIRT-1/FoxO3a signaling is crucial to induction of CYR-61 in synovial fibroblasts. PMID: 23239110
    11. Endogenous CCN1 regulates proliferation of VSMCs and neointimal hyperplasia. Inhibition of CCN1 may provide a promising strategy for the prevention of restenosis after vascular interventions [CCN1]. PMID: 18388330
    12. Galpha(12/13) regulate AP-1-dependent CYR61 induction in vascular smooth muscle, promoting migration, and they are upregulated with CYR61 in arteriosclerotic lesions. PMID: 21212405
    13. Suggest that CCN1, from the microenvironment of injured vessels, enhances reendothelialization via a direct action on endothelial progenitor cell differentiation, revealing a possible new mechanism underlying the process of vascular repair. PMID: 20830586
    14. Cyr61 induces a reversible epithelial-mesenchymal transition in gastric epithelial cells. PMID: 20458273
    15. CYR61 is synthesized in arterial smooth muscle walls during proliferative restenosis PMID: 11897702
    16. induction of cyr61 is important for neuronal cell death in the central nervous system hippocampal progenitor cells, and JNK activation, but not of p38, as well as the subsequent SRF phosphorylation are involved in cyr61 gene induction PMID: 12576482
    17. after severe glomerular damage in anti-Thy-1 glomerulonephritis, Cyr61 may be involved in glomerular remodeling as a factor secreted from podocytes to inhibit mesangial cell migration. PMID: 12707386
    18. Overexpression of the cyr61 gene in stably transfected rat SMC (smooth muscle cells) line A7r5 inhibited the proliferation and DNA and protein synthesis. Cyr61 can also act as a growth inhibitor in SMC of genetically hypertensive rats. PMID: 12861037
    19. In a rat balloon carotid arterial injury model, CYR61 was rapidly induced in VSMCs in the early stage of injury and remained elevated until 14 days. PMID: 17234971
    20. The dissimilar growth characteristics between high and low Cx43 expressors may be due to differential regulation of CCN3 by varying levels of Cx43 PMID: 18004727
    21. Data suggest that matrix protein CCN1 may play an important role in microenvironment-mediated biological properties of endothelial progenitor cells . PMID: 18755182
    22. THBS1, Cyr61 and CTGF were all over-expressed in esophageal squamous cell carcinoma. PMID: 19698122
    23. Cyr61 is an early marker of extracellular matrix remodeling in rat bladder cells PMID: 12217894

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    CCN family
  • 数据库链接：

    KEGG: rno:83476

    STRING: 10116.ENSRNOP00000019501

    UniGene: Rn.22129

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