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  4. Rat Syndecan-1/CD138(SDC1) ELISA Kit

Rat Syndecan-1/CD138(SDC1) ELISA Kit

文献引用 (4) 说明书下载
  • 中文名称：
    大鼠Syndecan-1/CD138(SDC1)酶联免疫试剂盒
  • 货号：
    CSB-E17115r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO定量检测大鼠Syndecan-1/CD138（SDC1）的酶联免疫试剂盒（货号：CSB-E17115r），采用夹心法原理设计，适用于血清、血浆及组织匀浆样本的检测。SDC1是跨膜硫酸乙酰肝素蛋白聚糖家族成员，作为细胞表面受体参与细胞粘附、迁移及信号传导调控，在炎症反应、肿瘤转移及组织修复等生理病理过程中具有重要作用。试剂盒检测灵敏度达0.312 ng/mL，线性范围为0.312-20 ng/mL，通过特异性抗体对实现目标蛋白的高精度捕获与定量分析。该产品可广泛应用于大鼠模型研究中SDC1表达的动态监测，例如探究炎症性疾病发展机制、肿瘤微环境中细胞间相互作用，或评估组织损伤修复过程中SDC1的调控功能，为分子机理研究及药物干预效果评价提供可靠工具。实验操作兼容常规实验室设备，支持科研人员高效获取重复性良好的数据。
  • 别名：
    Sdc1 ELISA Kit; Synd1 ELISA Kit; Syndecan-1 ELISA Kit; SYND1 ELISA Kit; CD antigen CD138 ELISA Kit
  • 缩写：
    SDC1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 88-102  
    1:2 Average % 101  
    Range % 95-104  
    1:4 Average % 90  
    Range % 83-94  
    1:8 Average % 94  
    Range % 87-99  
  • 回收率：
    The recovery of rat SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 102 94-107  
    EDTA plasma (n=4) 100 91-104  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.458 2.422 2.440 2.264  
    10 2.004 1.976 1.990 1.814  
    5 1.307 1.345 1.326 1.150  
    2.5 0.728 0.737 0.733 0.557  
    1.25 0.432 0.448 0.440 0.264  
    0.625 0.333 0.342 0.338 0.162  
    0.312 0.251 0.243 0.247 0.071  
    0 0.174 0.178 0.176    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Sdc1 antibodies

Sdc1 Proteins

靶点详情

  • 功能：
    Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
  • 基因功能参考文献：
    1. Plasma syndecan-1 and heparan sulfate correlate with microvascular glycocalyx degradation in hemorrhaged rats after different resuscitation fluids. PMID: 27037369
    2. down-regulation of Syndecan-1 could inhibit VEGF-VEGFR-2 signaling through regulating internalization of VEGFR-2. PMID: 27075925
    3. expression upregulated two months after renal transplantation PMID: 25972509
    4. data suggest that after renal transplantation loss of hepatic HS together with increased syndecan-1 shedding hampers lipoprotein binding and uptake by the liver contributing to dyslipidemia PMID: 25154787
    5. analysis of the molecular events that drive endocytosis of syndecan-1, a raft-dependent receptor and identification of its novel endocytic motif, MKKK PMID: 23525115
    6. The abnormal expression of HSPG may be an important molecular mechanism that leads to the occurrence and development of proteinuria in adriamycin-induced nephropathy rats. PMID: 20506637
    7. role in regulatory mechanism of apoptosis PMID: 11979781
    8. Syndecan-1 mRNA expression is not modulated by FSH as it would result from the antagonistic effects of increased intracellular cAMP and intracellular calcium levels. PMID: 12135485
    9. The major injury-related change, seen in injured brain and cultured glia, was an increase in 2-O-sulphated HS and increased syndecan-1, suggesting novel approaches to modulating scar formation. PMID: 18279312

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein. Secreted. Secreted, extracellular exosome.
  • 蛋白家族：
    Syndecan proteoglycan family
  • 数据库链接：

    KEGG: rno:25216

    STRING: 10116.ENSRNOP00000008582

    UniGene: Rn.11176

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