Rat TNF-α ELISA kit
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中文名称：大鼠肿瘤坏死因子α（TNF-α）酶联免疫试剂盒
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货号：CSB-E11987r-IS
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规格：96T/48T
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价格：￥3800/￥2500
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其他：
产品详情
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产品描述：CUSABIO试剂盒采用双抗体夹心ELISA技术对大鼠肿瘤坏死因子α（TNF-α）进行定量检测，适用于组织匀浆、细胞培养上清及支气管肺泡灌洗液样本分析。TNF-α作为重要的促炎细胞因子，在炎症反应、免疫调控及细胞凋亡中发挥核心作用，其表达水平与感染性疾病、自身免疫异常及神经退行性研究密切相关。该试剂盒检测灵敏度可达15.6 pg/mL，线性检测范围为15.6-1000 pg/mL，通过预包被特异性捕获抗体与标记检测抗体的协同作用，实现对目标蛋白的高精度定量。试剂盒配置96孔预包被板及全套实验组分，可满足炎症机制研究、药物干预效果评估、病理模型建立等科研需求，特别适用于败血症、关节炎、脑损伤等动物模型中炎症因子的动态监测。实验流程包含样本预处理、孵育反应及显色测定步骤，4小时内即可完成检测，为科研工作者提供稳定可靠的TNF-α定量分析工具。
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别名：Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
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缩写：
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Uniprot No.：
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种属：Rattus norvegicus (Rat)
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样本类型：tissue homogenates, cell culture supernates, bronchoalveolar lavage fluid
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检测范围：15.6 pg/mL-1000 pg/mL
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灵敏度：3.9 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
103
Range %
98-108
1:2
Average %
95
Range %
88-101
1:4
Average %
98
Range %
92-105
1:8
Average %
92
Range %
86-98
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回收率：
The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
94
89-100
EDTA plasma (n=4)
87
82-93
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
1000
2.717
2.634
2.675
2.487
500
1.476
1.467
1.471
1.283
250
0.853
0.908
0.881
0.693
125
0.608
0.629
0.619
0.431
62.5
0.395
0.381
0.388
0.200
31.2
0.281
0.293
0.287
0.099
15.6
0.218
0.207
0.213
0.025
0
0.191
0.185
0.188
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数据处理：
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货期：3-5 working days
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靶点详情
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功能：Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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亚细胞定位：Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
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蛋白家族：Tumor necrosis factor family
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数据库链接：
