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  4. Rat Tissue factor,TF ELISA Kit

Rat Tissue factor,TF ELISA Kit

文献引用 (4) 说明书下载
  • 中文名称：
    大鼠组织因子(TF)酶联免疫试剂盒
  • 货号：
    CSB-E07914r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO专为大鼠样本设计的高灵敏度组织因子(TF)酶联免疫检测试剂盒，采用双抗体夹心法原理，可对血清、尿液、细胞培养上清液、组织匀浆及细胞裂解液中的TF蛋白进行精确定量，检测范围覆盖31.25-2000 pg/mL。组织因子作为凝血级联反应的关键启动因子，在血栓形成、炎症反应及肿瘤血管生成等病理生理过程中发挥重要作用，其定量检测为研究凝血机制、炎症性疾病及肿瘤微环境调控提供重要工具。试剂盒适用于心血管疾病模型、肿瘤生物学研究、炎症相关实验等科研场景，通过检测不同样本类型中TF的动态变化，可深入探究其在疾病发生发展中的作用机制，或用于药物干预实验中生物标志物的效果评估。该试剂盒具备操作简便、重复性佳的特点，标准曲线线性相关系数R²＞0.99，最低检测限达15.6 pg/mL，满足科研实验中对低丰度靶标蛋白的定量需求，为实验室开展大鼠模型相关研究提供可靠的技术支持。
  • 别名：
    F3 ELISA Kit; Cf3 ELISA Kit; Tissue factor ELISA Kit; TF ELISA Kit; Coagulation factor III ELISA Kit; CD antigen CD142 ELISA Kit
  • 缩写：
    F3
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, urine, cell culture supernates, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Blood Coagulation
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %102
    Range %91-106
    1:2Average %90
    Range %85-96
    1:4Average %93
    Range %89-100
    1:8Average %99
    Range %93-108
  • 回收率：
    The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8884-94
    EDTA plasma (n=4)10395-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.297 2.231 2.264 2.081
    10001.573 1.471 1.522 1.339
    5000.999 0.924 0.962 0.779
    2500.618 0.593 0.606 0.423
    1250.405 0.425 0.415 0.232
    62.50.293 0.310 0.302 0.119
    31.250.249 0.264 0.257 0.074
    00.177 0.188 0.183 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Tissue factor family
  • 数据库链接：

    STRING: 10116.ENSRNOP00000015836

    UniGene: Rn.9980

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