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  4. Rat Tumor necrosis factor β,TNF-β ELISA KIT

Rat Tumor necrosis factor β,TNF-β ELISA KIT

文献引用 (13) 说明书下载
  • 中文名称：
    大鼠肿瘤坏死因子β(TNF-β)酶联免疫试剂盒
  • 货号：
    CSB-E07379r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO专用于科研领域的大鼠肿瘤坏死因子β（TNF-β）酶联免疫检测试剂盒（货号：CSB-E07379r），基于双抗体夹心法定量分析血清、血浆及组织匀浆样本中的TNF-β含量。TNF-β作为肿瘤坏死因子超家族成员，在炎症反应、免疫调节及细胞凋亡等生物学过程中发挥重要作用，是研究免疫相关疾病、肿瘤发生机制及药物筛选的重要靶点。试剂盒检测范围为7.8-500 pg/mL，最低检测限达7.8 pg/mL，可在约3小时内完成检测流程，具备高灵敏度和宽线性范围的优势。其优化的实验体系支持多种生物样本类型，尤其适用于动物模型研究中TNF-β的动态监测，例如炎症性疾病建模、免疫调节实验或抗肿瘤药物作用评估等场景。通过标准化的操作流程，该试剂盒能够稳定检测微量目标蛋白，为科研人员探索TNF-β在病理生理过程中的分子机制提供可靠工具。
  • 别名：
    Lta ELISA Kit; Tnfb ELISA Kit; Tnfsf1 ELISA Kit; Lymphotoxin-alpha ELISA Kit; LT-alpha ELISA Kit; TNF-beta ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit
  • 缩写：
    TNF-β
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 101
    Range % 96-105
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 87
    Range % 83-94
    1:8 Average % 96
    Range % 91-102
  • 回收率：
    The recovery of rat TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 99108
    EDTA plasma (n=4) 88 84-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    500 2.632 2.765 2.699 2.614
    250 2.212 2.302 2.257 2.172
    125 1.545 1.577 1.561 1.476
    62.5 0.765 0.771 0.768 0.683
    31.2 0.411 0.423 0.417 0.332
    15.6 0.257 0.264 0.261 0.176
    7.8 0.176 0.185 0.181 0.096
    0 0.084 0.085 0.085 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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靶点详情

  • 功能：
    Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.
  • 基因功能参考文献：
    1. Adrenomedullin can increase the expression of TNF-beta and TNFR in renal early trauma. PMID: 20092780
    2. TNFbeta-so far only known to be produced by lymphocytes-was demonstrated in alveolar macrophages, bronchial epithelial cells, vascular smooth muscle cells and endothelial cells at the protein and the message level PMID: 12849708
  • 亚细胞定位：
    Secreted. Membrane.
  • 蛋白家族：
    Tumor necrosis factor family
  • 数据库链接：

    KEGG: rno:25008

    STRING: 10116.ENSRNOP00000001111

    UniGene: Rn.160577

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