Rat Vascular Endothelial cell Growth Factor D,VEGF-D ELISA kit
产品详情
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产品描述：
CUSABIO专为大鼠样本设计的血管内皮细胞生长因子D（VEGF-D）定量检测试剂盒，采用双抗体夹心法原理，可精准检测血清、血浆及组织匀浆中的VEGF-D蛋白水平。VEGF-D作为血管内皮生长因子家族成员，在血管生成、淋巴管形成及肿瘤转移等生理病理过程中发挥关键调控作用，其定量分析对心血管疾病、肿瘤生物学等领域的机制研究具有重要意义。试剂盒检测灵敏度达0.312 ng/mL，线性范围覆盖0.312-20 ng/mL，标准品采用梯度冻干粉形式，配套提供预包被96孔板、检测抗体及显色系统，操作流程标准化，3.5小时内即可完成检测。该试剂盒适用于实验动物模型（如肿瘤、缺血性损伤等）中VEGF-D的动态监测，可兼容多种生物样本类型，为研究组织微环境变化、药物干预效果或基因表达调控提供可靠工具，实验结果需通过酶标仪在450nm波长下读取，建议同步使用标准曲线进行数据校正以确保准确性。
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别名：
Vegfd ELISA kit; FigfVascular endothelial growth factor D ELISA kit; VEGF-D ELISA kit; c-Fos-induced growth factor ELISA kit; FIGF ELISA kit
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缩写：
FIGF
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Uniprot No.：
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种属：
Rattus norvegicus (Rat)
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样本类型：
serum, plasma, tissue homogenates
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检测范围：
0.312 ng/mL-20 ng/mL
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灵敏度：
0.078 ng/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Cardiovascular
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 87 | | |
| Range % | 83-92 | | |
| 1:2 | Average % | 101 | | |
| Range % | 97-105 | | |
| 1:4 | Average % | 91 | | |
| Range % | 85-97 | | |
| 1:8 | Average % | 97 | | |
| Range % | 91-103 | | |
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回收率：
| The recovery of rat VEGF-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 91 | 86-95 | | |
| EDTA plasma (n=4) | 98 | 94-102 | | |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 1.932 | 1.837 | 1.885 | 1.738 | | | | 10 | 1.456 | 1.566 | 1.511 | 1.364 | | | | 5 | 1.088 | 1.042 | 1.065 | 0.918 | | | | 2.5 | 0.713 | 0.707 | 0.710 | 0.563 | | | | 1.25 | 0.492 | 0.481 | 0.487 | 0.340 | | | | 0.625 | 0.358 | 0.342 | 0.350 | 0.203 | | | | 0.312 | 0.272 | 0.278 | 0.275 | 0.128 | | | | 0 | 0.148 | 0.146 | 0.147 | | | |
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数据处理：
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货期：
3-5 working days
靶点详情
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功能：
Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-3 (Flt4) receptor.
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基因功能参考文献：
- Expression of VEGF-D and its receptor, VEGFR-3, are enhanced in various cell types in the infarcted heart. PMID: 22818386
- Adenovirus encoding vascular endothelial growth factor-D induces tissue-specific vascular patterns in vivo. PMID: 12036873
- Overexpression of VEGF-D is associated with Gliosarcomas PMID: 16533777
- VEGF-D expression may have a role in interleukin (IL)-6 expression and transcription factor AP-1 activation in pancreatic acinar cells. PMID: 17404025
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亚细胞定位：
Secreted.
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蛋白家族：
PDGF/VEGF growth factor family
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组织特异性：
Highly expressed in the spleen, kidney, lung, tongue, ovary and mammary gland.
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数据库链接：
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