Your Good Partner in Biology Research

Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

  • 中文名称:
    大鼠β淀粉样蛋白1-42(Aβ1-42)酶联免疫试剂盒
  • 货号:
    CSB-E10786r
  • 规格:
    96T/48T
  • 价格:
    ¥3900/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO大鼠β淀粉样蛋白1-42(Aβ1-42)酶联免疫检测试剂盒(货号:CSB-E10786r),采用双抗体夹心法原理实现定量检测,适用于血清、血浆及组织匀浆样本中Aβ1-42含量的精准分析。β淀粉样蛋白1-42作为阿尔茨海默病等神经退行性疾病的重要病理标志物,其异常聚集形成的斑块是相关疾病机制研究的核心对象。本试剂盒检测灵敏度为0.9 pg/mL,线性范围覆盖0.9-60 pg/mL,实验全程仅需3.5小时,包含预包被96孔板、重组蛋白标准品、生物素标记检测抗体、链霉亲和素-HRP复合物及显色底物等核心组分,所有组分需在-20°C避光保存且有效期为6个月。该试剂盒适用于神经科学领域的基础研究,如阿尔茨海默病动物模型中Aβ1-42的动态变化观察、药物干预效果评价或病理机制探索,其宽泛的样本兼容性可满足脑组织、血液等多种生物样本的检测需求。实验操作时需注意样本可能需预先稀释,推荐通过预实验确定最佳稀释比例以保证检测准确性。
  • 别名:
    Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
  • 缩写:
    Aβ1-42
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.9 pg/mL-60 pg/mL
  • 灵敏度:
    0.225 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %87
    Range %83-90
    1:2Average %94
    Range %90-98
    1:4Average %101
    Range %97-104
    1:8Average %96
    Range %93-99
  • 回收率:
    The recovery of rat Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)10095-105
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    602.281 2.274 2.278 2.136
    301.599 1.576 1.588 1.446
    150.967 0.924 0.946 0.804
    7.50.538 0.528 0.533 0.391
    3.750.395 0.371 0.383 0.241
    1.80.321 0.312 0.317 0.175
    0.90.235 0.250 0.243 0.101
    00.141 0.143 0.142  
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

相关问答

 常见问题解答
Q:

我们的实验室打算购买β淀粉样蛋白1-42,Aβ1-42 ELISA试剂盒,货号是CSB-E10786r。但是,我们使用的是含有SDS的RIPA缓冲液来裂解组织,同时我们也用相同的RIPA缓冲液和1x PBS稀释了样品。我想知道这样做是否会影响试剂盒的效果

A:
对于ELISA检测,一般不建议使用含有SDS的缓冲液处理样品。这是因为SDS可以破坏分子与分子间的氢键,即破坏蛋白质的二级和三级结构,并可能影响ELISA的检测。由于您的样品已经被处理过,我们只能建议您先使用处理后的样品进行梯度预实验。我们以前没有这样做过,所以我们只能建议你试一试。