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中文名称：大鼠血管紧张素1-7(Ang1-7)酶联免疫试剂盒
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货号：CSB-E14241r
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO定量检测大鼠血清、血浆及组织匀浆样本中血管紧张素1-7（Ang1-7）的酶联免疫试剂盒（货号：CSB-E14241r），采用双抗体夹心法原理，检测灵敏度达18.75 pg/mL，线性范围为18.75-1200 pg/mL。Ang1-7作为肾素-血管紧张素系统的重要活性肽，参与调节心血管功能、炎症反应及组织修复等生理过程，在高血压、糖尿病肾病等疾病机制研究中具有重要价值。试剂盒通过特异性抗体对实现目标分子的高精度捕获与定量分析，适用于基础科研中评估心血管疾病模型、代谢紊乱或器官损伤等实验条件下Ang1-7的动态变化。配套提供预包被微孔板、标准品及检测试剂，支持多样本类型检测，操作流程标准化，满足实验室对生物活性分子定量分析的需求。
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缩写：Ang1-7
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, tissue homogenates
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检测范围：18.75 pg/mL-1200 pg/mL
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灵敏度：4.68 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Cardiovascular
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 94 Range % 80-105 1:2 Average % 96 Range % 85-104 1:4 Average % 102 Range % 92-114 1:8 Average % 99 Range % 80-110 -
回收率：
The recovery of rat Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 83-112 EDTA plasma (n=4) 95 90-102 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1200 3.121 3.129 3.125 2.986 600 2.532 2.522 2.527 2.388 300 1.739 1.765 1.752 1.613 150 1.050 1.041 1.046 0.907 75 0.623 0.642 0.633 0.494 37.5 0.435 0.447 0.441 0.302 18.75 0.265 0.254 0.260 0.121 0 0.135 0.143 0.139 -
数据处理：
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货期：3-5 working days
引用文献
- Chicken Muscle-Derived ACE2 Upregulating Peptide VVHPKESF Inhibits Angiotensin II-Stimulated Inflammation in Vascular Smooth Muscle Cells via the ACE2/Ang (1–7)/MasR Axis H Fan,Journal of agricultural and food chemistry,2023
- Biomimetic Nanoparticle-Mediated Target Delivery of Hypoxia-Responsive Plasmid of Angiotensin-Converting Enzyme 2 to Reverse Hypoxic Pulmonary Hypertension R Yuan,ACS nano,2023
- The Expression of RAAS Key Receptors, Agtr2 and Bdkrb1, Is Downregulated at an Early Stage in a Rat Model of Wolfram Syndrome M Punapart,Genes,2021
- CoQ10 augments candesartan protective effect against tourniquet-induced hind limb ischemia-reperfusion: involvement of non-classical RAS and ROS pathways AS Awad,Saudi Pharmaceutical Journal,2021
- Vitamin A deficiency indicating as low expression of LRAT may be a novel biomarker of primary hypertension X Liang,Journal Clinical and Experimental Hypertension,2020
- The contribution of chymase-dependent formation of AngII to cardiac dysfunction in metabolic syndrome of young rats: roles of fructose and EETs G Froogh,Am J Physiol Heart Circ Physiol,2020
- Dynamic Variation of RAS on Silicotic Fibrosis Pathogenesis in Rats Zhang BN, et al,Current Medical Science,2019
- Protective Effect of Angiotensin (1-7) on Silicotic Fibrosis in Rats Zhang BN, et al,Biomedical and Environmental Sciences,2019
- Blood pressure lowering effect of Ficus deltoidea var kunstleri in spontaneously hypertensive rats: possible involvement of renin-angiotensin-aldosterone system, endothelial function and anti-oxidant system Azis NA, et al,Molecular Biology Reports,2019
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相关问答
■ 常见问题解答
Q:
What are host animals of antibodies in the kit? Are the antibodies affinity purified?
A:
Thanks for your inquiry.Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody. It is affinity purified. The standard is synthetic peptides. Pls let me know if you have any further questions. Thank you.
