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  4. Rat calcitonin gene related peptide,CGRP ELISA Kit

Rat calcitonin gene related peptide,CGRP ELISA Kit

文献引用 (13) 说明书下载
  • 中文名称：
    大鼠降钙素基因相关肽(CGRP)酶联免疫试剂盒
  • 货号：
    CSB-E08211r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠降钙素基因相关肽(CGRP)酶联免疫试剂盒（CSB-E08211r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CGRP含量。降钙素基因相关肽（CGRP）是一种神经肽。背景上，它在疼痛传导等方面起着重要作用，尤其与偏头痛发病机制关联密切。研究机制上，主要聚焦于其受体及信号通路，通过阻断CGRP及其受体，有望开发出治疗偏头痛等疼痛性疾病的药物。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于科研场景中CGRP的定量分析，例如神经生物学研究中的痛觉传导机制探索、心血管疾病模型中的血管舒缩功能评估，或药理学实验中对CGRP调控类化合物作用的体外验证，为相关疾病机制研究及药物开发评估提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Calcb ELISA Kit; Calcitonin gene-related peptide 2 ELISA Kit; Beta-type CGRP ELISA Kit; Calcitonin gene-related peptide II ELISA Kit; CGRP-II ELISA Kit
  • 缩写：
    CGRP
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-95
    1:2Average %94
    Range %87-101
    1:4Average %90
    Range %86-106
    1:8Average %95
    Range %82-100
  • 回收率：
    The recovery of rat CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
    EDTA plasma (n=4)9488-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.742 2.725 2.734 2.562
    2002.137 2.183 2.160 1.988
    1001.384 1.481 1.433 1.261
    500.863 0.806 0.835 0.663
    250.452 0.475 0.464 0.292
    12.50.298 0.292 0.295 0.123
    6.250.203 0.214 0.209 0.037
    00.171 0.173 0.172 　
  • 本试剂盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat CGRP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled CGRP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Calcb Proteins

相关问答

■ 常见问题解答
Q:

Pls inform me the sequence of the standard in this kit

A:
Thanks for your inquiry. This kit is designed against full length protein.Pls refer to https://www.uniprot.org/uniprot/P10093.The standard in it is 293 cell-derived recombinant protein.The sequence of the standard is confidential.Sorry we can not disclose it. Hope you could understand.

靶点详情

  • 功能：
    CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
  • 基因功能参考文献：
    1. results indicate that calcitonin gene-related peptide-B receptors are present in rat placenta and that receptor levels are higher with gestational age and lower at term labor (Calcb) PMID: 12297551
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calcitonin family
  • 数据库链接：

    KEGG: rno:171519

    STRING: 10116.ENSRNOP00000014764

    UniGene: Rn.88080

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