Rat cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) ELISA kit

A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives.

1. lnc-HC negatively regulates cholesterol metabolism within hepatocytes through physical interaction with hnRNPA2B1. By further binding to the target messenger RNA of Cyp7a1 or Abca1, the lnc-HC-hnRNPA2B1 complex decreases expressions of the two genes that are implicated in cellular cholesterol excretion. PMID: 26663205
2. Administration of glucocorticoids (GCs) enhanced liver bile acid uptake by increasing the expression of the major hepatocyte basolateral bile transporter (Ntcp) in rats. The reduced expression of a bile acid synthesis rate-controlling enzyme, Cyp7a1, suggests that treatment with GCs suppressed hepatic bile acid synthesis. PMID: 27133208
3. Dietary fats and glucomannan plus spirulina added to pork significantly alters Cyp7a1 expression in Zucker rats. PMID: 26475369
4. Ginsenosides promoted liver cholesterol metabolism due to upregulation of CYP7A1. PMID: 23108811
5. Portal blood FGF15/19 levels remain unchanged while the corresponding ileal mRNA and liver CYP7A1 levels change significantly in cholesterol fed rats. PMID: 23852734
6. Capsaicinoids consumption down-regulated the mRNA levels of HMG-CoA reductase and CYP7A1 in the cholesterol-free diet group but up-regulated the CYP7A1 level in the cholesterol-enriched diet group. PMID: 23617777
7. insulin-dependent suppression results in impairment of the hepatic cholesterol/bile acid metabolisms PMID: 21817852
8. Maternal protein restriction elevates cholesterol in adult rat offspring due to repressive changes in histone modifications at the cholesterol 7alpha-hydroxylase promoter PMID: 21372147
9. Cytochrome P450 7A1 cholesterol 7alpha-hydroxylation: individual reaction steps in the catalytic cycle and rate-limiting ferric iron reduction. PMID: 21147774
10. essential for heart and skeletal muscle function PMID: 20215401
11. The mRNA and protein expressions of SHP and CYP7A1 were increased in livers of pregnant rats with intrahepatic cholestasis. PMID: 18578998
12. Cyp7a1 activity is modified after chronic ingestion of depleted uranium in the rat. PMID: 20362056
13. The increased recruitment of LXRalpha, a Cyp7a1 stimulatory pathway, and decreased expression of FGF15 and phosphorylated Erk1/2, a Cyp7a1 repressive pathway, combined to increase Cyp7a1 expression during lactation. PMID: 19957370
14. LXR alpha is the dominant regulator of CYP7A1 transcription PMID: 12054605
15. transcription suppressed by alpha1-antitrypsin peptide via interaction with AFP PMID: 12223476
16. Data reveal a fundamental difference in the regulation of CYP7A1 in rodent and human hepatocytes. PMID: 12554795
17. ARP-1/COUP-TFII acts as a transcription activator of CYP7A1. PMID: 12777384
18. results suggest that the mechanisms of regulation of these bile acid metabolizing enzymes are significantly different during liver regeneration PMID: 16081591
19. These results suggest that the cyp7A1 gene plays an important role in development of hypercholesterolemia in Prague hereditary hypercholesterolemic rats. PMID: 18226361
20. Increased Cyp7a1 expression inlactating rats provides a mechanism for the increase in the bile acid pool. PMID: 18292185
21. The effect of osthole on protein expression of PPARalpha/gamma and related target molecules such as CYP7A and DGAT protein expression in the liver of hyperlipidemic fatty liver rats. PMID: 18696335
22. A relationship between the decreased expression of hepatic CYP7A1 mRNA and protein and dilated ER may contribute to hypercholesterolemia in chronic hypothyroidism. PMID: 19788618

显示更多

收起更多

Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.

Cytochrome P450 family

Detected in liver (at protein level). Liver.

KEGG: rno:25428

STRING: 10116.ENSRNOP00000012819

UniGene: Rn.10737