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  4. Rat heparin-binding EGF-like growth factor (HBEGF) ELISA kit

Rat heparin-binding EGF-like growth factor (HBEGF) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    大鼠肝素结合EGF样生长因子(HBEGF)酶联免疫试剂盒
  • 货号：
    CSB-EL010154RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO定量检测大鼠血清、血浆及组织匀浆中肝素结合EGF样生长因子（HBEGF）的酶联免疫试剂盒，采用双抗体夹心技术原理，检测灵敏度高，特异性强。HBEGF是一种参与细胞增殖、分化和组织修复的关键生长因子，在胚胎发育、血管生成及肿瘤微环境调控中发挥重要作用。试剂盒检测范围覆盖6.25至400皮克/毫升，标准曲线线性优异，支持多样本类型检测，实验流程包含预包被抗体、生物素标记检测抗体及链霉亲和素-HRP系统，操作步骤标准化，可在4.5小时内完成检测。该产品适用于科研领域研究HBEGF在肝损伤、心血管疾病或肿瘤模型中的表达调控机制，例如通过检测肝纤维化模型中组织匀浆HBEGF水平变化，或分析药物干预后血浆因子动态变化，为探索HBEGF相关信号通路及疾病机理提供可靠工具。
  • 别名：
    Hbegf; Dtr; Hegfl; Proheparin-binding EGF-like growth factor
  • 缩写：
    HBEGF
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HBEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 104
    Range % 91-108
    1:2 Average % 100
    Range % 91-105
    1:4 Average % 94
    Range % 87-99
    1:8 Average % 98
    Range % 90-103
  • 回收率：
    The recovery of rat HBEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 81-95
    EDTA plasma (n=4) 102 95-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    400 2.727 2.586 2.657 2.537
    200 2.229 2.179 2.204 2.084
    100 1.452 1.428 1.440 1.320
    50 0.739 0.727 0.733 0.613
    25 0.423 0.411 0.417 0.297
    12.5 0.268 0.261 0.265 0.145
    6.25 0.182 0.175 0.179 0.059
    0 0.118 0.122 0.120 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Hbegf Proteins

靶点详情

  • 功能：
    Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
  • 基因功能参考文献：
    1. Heterotrimeric G proteins directly regulate membrane-localized MMP14/MT1-MMP, resulting in HB-EGF release and EGFR transactivation. PMID: 25759388
    2. HB-EGF promotes hepatic stellate cell proliferation via activation of the EGFR and ErbB4 receptors PMID: 24303948
    3. FAK phosphorylation and FAK-mediated signal transduction play essential roles in HB-EGF-mediated intestinal epithelial cell migration and adhesion. PMID: 24703506
    4. Intraluminal administration of HB-EGF reduces the severity of intestinal congestion/repurfusion(C/R) injury in rats. PMID: 23534509
    5. HB-EGF induces cardiomyocyte hypertrophy through an EGFR-ERK5-MEF2A-COX-2 pathway. PMID: 21244855
    6. There were no significant changes in HB-EGF mRNA or protein expression in either the plantaris or the soleus in response to functional overload. PMID: 20696782
    7. HB-EGF contributes to vascular remodeling. PMID: 11882602
    8. HB-EGF expressed in proximity to developing mesencephalic dopaminergic neurons promotes their survival in vitro. HB-EGF may be an important molecule for developing dopaminergic neurons of the ventral midbrain. PMID: 11956950
    9. data indicate HB-EGF gene expression can be regulated by Cdx2 in intestinal epithelial (IEC-6) cells PMID: 12223343
    10. Data suggest that the interaction between HB-EGF and EGFR transactivation is closely related to the proliferation of cardiac fibroblasts and cardiac remodeling after myocardial infarction. PMID: 12237129
    11. HB-EGF mRNA expression and protein production in intestine undergoing ischemia/reperfusion injury PMID: 12746188
    12. Chelerythrine stimulated production of reactive oxygen species, and antioxidants prevented chelerythrine-induced HB-EGF shedding, suggesting that the production of intracellular peroxides is necessary for this event PMID: 15490481
    13. analysis of clearance of HB-EGF in adult and newborn rats PMID: 16364500
    14. 5-HT2A receptor induces ERK phosphorylation and proliferation through TACE enzyme activation and HB-EGF shedding in mesangial cells PMID: 16737974
    15. extracellular superoxide dismutase is related to the epidermal growth factor (EGF) signaling in two ways, competition for HSPG with heparin-binding-EGF and as an ROS scavenger PMID: 16753836
    16. It is suggested that the increased expression of HB-EGF and the inhibition of glial cell swelling may be parts of a protective role of HB-EGF in the ischemic retina. PMID: 16806064
    17. Juxtacrine activation of EGFR by membrane-anchored HB-EGF may play an important role in the regulation of tight junction proteins and transepithelial resistance. PMID: 17855771
    18. Collectively, these data demonstrate that membrane-anchored HB-EGF is targeted to the inner nuclear membrane via a retrograde membrane trafficking pathway. PMID: 18299347
    19. Pro-inflammatory stimuli induce the release of HB-EGF from mesenchyme cells, which stimulates DNA-replication in initiated/premalignant hepatocytes PMID: 18929421
    20. rapid HA accumulation after epidermal wounding occurs through a mechanism requiring cleavage of HB-EGF and activation of EGFR signaling. PMID: 19225541
    21. mechanical stretch promotes fetal type II cell differentiation via ectodomain shedding of HB-EGF and TGF-alpha PMID: 19237431
    22. Hbegf is a potent dilator of terminal mesenteric arterioles. PMID: 19389413

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  • 亚细胞定位：
    [Heparin-binding EGF-like growth factor]: Secreted, extracellular space. Note=Mature HB-EGF is released into the extracellular space and probably binds to a receptor.; [Proheparin-binding EGF-like growth factor]: Cell membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Most abundant in skeletal muscle, lung, spleen brain and heart.
  • 数据库链接：

    KEGG: rno:25433

    STRING: 10116.ENSRNOP00000025157

    UniGene: Rn.10148

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