Rat inositol 1,4,5,-trisphosphate(IP3) ELISA kit
产品详情
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产品描述：
大鼠1,4,5-三磷酸肌醇(IP3)酶联免疫试剂盒 （CSB-E13004r）为竞争法ELISA试剂盒，定量检测血清、血浆、tissue
匀浆、组织培养上清液、细胞裂解物样本中的IP3含量。IP3（肌醇三磷酸）是一种重要的细胞信号分子，由磷脂酶C（PLC）水解磷脂酰肌醇-4,5-二磷酸（PIP2）产生。它作为第二信使，通过结合内质网上的IP3受体，触发钙离子释放，从而调节细胞内钙信号，影响多种细胞反应，如平滑肌收缩、血小板聚集等。研究IP3信号转导机制有助于理解相关疾病的发生和发展，为疾病治疗提供新靶点。试剂盒检测范围为5 pg/mL-1000 pg/mL，该产品适用于神经生物学、心血管疾病模型、药物作用机制等研究领域，可用于探索细胞内信号通路、评估化合物干预效果。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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缩写：
IP3
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种属：
Rattus norvegicus (Rat)
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样本类型：
serum, plasma, tissue homogenates, cell culture supernates, cell lysates
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检测范围：
5 pg/mL-1000 pg/mL
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灵敏度：
2.5 pg/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Signal Transduction
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测定原理：
quantitative
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测定方法：
Competitive
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:2 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:4 |
Average % |
96 |
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| Range % |
87-102 |
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| 1:8 |
Average % |
97 |
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| Range % |
91-104 |
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回收率：
| The recovery of rat IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
91-105 |
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| EDTA plasma (n=4) |
95 |
92-98 |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 1000 |
0.141 |
0.143 |
0.142 |
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| 250 |
0.270 |
0.261 |
0.266 |
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| 80 |
0.423 |
0.440 |
0.432 |
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| 20 |
0.744 |
0.759 |
0.752 |
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| 5 |
1.156 |
1.162 |
1.159 |
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| 0 |
2.199 |
2.214 |
2.207 |
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数据处理：
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货期：
3-5 working days
引用文献
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Selective Inhibition of P2Y1 and P2Y12 Receptor Signal Pathways in Platelet Aggregation in Transgenic Cell Lines and Rats by Potassium 2-(1-Hydroxypentyl)-Benzoate, Puerarin and Salvianolic Acid B
Y Li, W Wang, J Cai, N Feng, S Xu,CNS Neuroscience & Therapeutics,2024
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Anandamide modulates WNT-5A/BCL-2, IP3/NFATc1, and HMGB1/NF-κB trajectories to protect against mercuric chloride-induced acute kidney injury
DM Abdallah,Scientific reports,2023
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Nitric Oxide Linked to mGluR5 Upregulates BDNF Synthesis by Activating MMP2 in the Caudate and Putamen after Challenge Exposure to Nicotine in Rats
J Kim,International journal of molecular sciences,2022
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Kaempferol suppresses acetaminophen-induced liver damage by upregulation/activation of SIRT1
Nora Abdullah AlFaris,Pharmaceutical Biology,2021
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Amphetamine-induced conditioned place preference and changes in mGlu1/5 receptor expression and signaling in the rat medial prefrontal cortex
Mao LM, et al,Neuroscience,2018
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Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling
Ji Eun Kim, et al,International Journal
of Molecular
Medicine,2018
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Molecular mechanism of Danshensu on platelet antiaggregation
Chen Yu.et al,American Institute of Physics,2018
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Alterations in mGlu5 receptor expression and function in the striatum in a rat depression model
Mao LM.et al,Journal of Neurochemistry,2018
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Melatonin Promotes Brain-Derived Neurotrophic Factor (BDNF) Expression and Anti-Apoptotic Effects in Neonatal Hemolytic Hyperbilirubinemia via a Phospholipase
Yong Luo.et al,Medical Science Monitor 23,2017
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Protective role of Pollen Typhae total flavone against the palmitic acid-induced impairment of glucose-stimulated insulin secretion involving GPR40 signaling in INS-1 cells
Feng XT.et al,Int J Mol Med,2017
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Uridine stimulate laxative effect in the loperamide-induced constipation of SD rats through regulation of the mAChRs signaling pathway and mucin secretion
Ji Eun Kim,.et al,BMC Genet.,2017
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Effects of five candidate laxatives derived from Liriope platyphylla on the 5-HT receptor signaling pathway in three cell types present in the transverse colon
Ji Eun Kim.et al,Molecular Medicine Reports,2016
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A long-acting β2-adrenergic agonist increases the expression of muscarine cholinergic subtype
Liu YH.et al,Mol Med Rep.,2015
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A long-acting ß2-adrenergic agonist increases the expression of muscarine cholinergic subtype-3 receptors by activating the ß2-adrenoceptor cyclic adenosine monophosphate signaling pathway in airway smooth muscle cells.
Yuan-Hua Liu et al,Molecular Medicine reports,2015
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Lipid peroxidation is essential for phospholipase C activity and IP3 related calcium signal
Domijan AM et al,J Cell Sci,2013
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Effect of FSH receptor-binding inhibitor-8 on FSH-mediated granulosa cell signaling and proliferation.
Navalakhe RM et al,Chem Biol Drug Des,2013
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