Rat lipolysaccharide binding protein,LBP ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO高灵敏度大鼠脂多糖结合蛋白（LBP）酶联免疫检测试剂盒（货号：CSB-E11184r），采用双抗体夹心技术原理，可定量检测血清、血浆、细胞培养上清及组织匀浆样本中的LBP含量，检测范围为62.5-4000 pg/mL。LBP作为天然免疫系统中的关键调节蛋白，通过与细菌内毒素脂多糖（LPS）结合参与宿主防御反应，在炎症调控、感染性疾病机制等基础研究中具有重要价值。该试剂盒基于特异性抗体对实现精准检测，实验过程兼容常规实验室操作，适用于评估败血症、肠道炎症等病理模型中LBP的动态变化，或用于免疫调节药物开发中生物标志物的定量分析。试剂盒提供预包被酶标板及完整检测组分，支持科研人员在体外实验中系统研究LBP在炎症反应、细菌感染等生理病理过程中的作用机制，为相关领域的分子机制探索提供可靠工具。
别名：

LbpLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
缩写：

LBP
Uniprot No.：

Q63313
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	85
	Range %	81-89
1:10	Average %	94
	Range %	91-98
1:20	Average %	91
	Range %	85-97
1:40	Average %	86
	Range %	82-89

回收率：

The recovery of rat LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
亚细胞定位：

Secreted. Cytoplasmic granule membrane.
蛋白家族：

BPI/LBP/Plunc superfamily, BPI/LBP family
组织特异性：

Detected in lung, liver, heart and kidney.
数据库链接：

STRING: 10116.ENSRNOP00000019787

UniGene: Rn.48863