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  4. Rat low density lipoprotein receptor,LDLR ELISA Kit

Rat low density lipoprotein receptor,LDLR ELISA Kit

说明书下载
  • 中文名称：
    大鼠低密度脂蛋白受体(LDLR)酶联免疫试剂盒
  • 货号：
    CSB-E13545r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO双抗体夹心法ELISA试剂盒，专用于定量检测大鼠血清、血浆及组织匀浆样本中的低密度脂蛋白受体（LDLR）水平。LDLR作为介导胆固醇内吞的关键膜蛋白受体，在脂质代谢调控中发挥核心作用，其表达水平与高脂血症、动脉粥样硬化等病理机制密切相关。试剂盒检测灵敏度高，线性范围为7.8-500 pg/mL，可精确检测微量样本中的LDLR含量，适用于基础科研中探究高胆固醇饮食模型、心血管疾病动物模型的LDLR动态变化，以及药物干预对脂质代谢通路影响的机制研究。实验操作采用预包被抗体的96孔板系统，配套标准品、生物素标记检测抗体及显色底物，完成样本孵育、洗涤和比色分析后，通过标准曲线实现目标蛋白的精准定量。本试剂盒适用于肝脏、血管等组织样本与体液样本的平行检测，为脂质代谢相关研究提供可靠工具。
  • 别名：
    Ldlr ELISA Kit; Low-density lipoprotein receptor ELISA Kit; LDL receptor ELISA Kit
  • 缩写：
    LDLR
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LDLR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:200 Average % 85
    Range % 80-89
    1:400 Average % 102
    Range % 98-108
    1:800 Average % 90
    Range % 85-94
    1:1600 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of rat LDLR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 88 84-92
    EDTA plasma (n=4) 93 89-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    500 2.512 2.475 2.494 2.355
    250 2.247 2.196 2.222 2.083
    125 1.803 1.689 1.746 1.607
    62.5 1.189 1.227 1.208 1.069
    31.2 0.687 0.713 0.700 0.561
    15.6 0.415 0.392 0.404 0.265
    7.8 0.298 0.289 0.294 0.155
    0 0.136 0.141 0.139 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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Ldlr Proteins

靶点详情

  • 功能：
    Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.
  • 基因功能参考文献：
    1. LDLR is a relevant receptor for CNS drug delivery via receptor-mediated transcytosis and that the peptide vectors we developed have the potential to transport drugs PMID: 28108572
    2. Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol accumulation via increased LDL cholesterol uptake into the liver with resulting persistent increases in hepatic cholesterol accumulation. PMID: 27401460
    3. Compared with the cholesterol-enriched restructured pork group, silicon-enriched restructured pork rats had lower VLDL compound concentrations and lower VLDL oxidation but greater Ldlr expression. PMID: 26246324
    4. The LDL receptor could be located at once in clathrin-coated pits and caveolae. PMID: 24530906
    5. LDLR mutant rats are a useful novel animal model of hypercholesterolemia and atherosclerosis. PMID: 22293196
    6. A tissue-specific dysregulation of LDLr is demonstrated that is age- and gender-dependent. PMID: 21792919
    7. expression of LDLR was increased in the hippocampus of stroke-prone spontaneously hypertensive rats and was seen in the luminal and abluminal cytoplasmic membranes and vesicular structures of endothelial cells PMID: 20458492
    8. expression pattern of LDL-R in brain microvessels endothelial cells and its modulation by astrocytes PMID: 12095612
    9. cloning and sequencing of the rat LDL receptor 5'-flanking region revealed the presence of several putative cis-acting elements PMID: 16647292
    10. Retina is capable of rapid uptake of circulating LDL via LDLR-mediated process primarily occurring in retinal pigment epithelium and also possibly Muller cells. PMID: 17110914
    11. Caloric restriction affects Ldlr involved in age-related hypercholesterolemia. PMID: 17203467
    12. LDLR-mediated pathway was involved in the endocytosis of Poly(methoxypolyethyleneglycol cyanoacrylate-co-hexadecylcyanoacrylate) (PEG-PHDCA) nanoparticles by RBEC. PMID: 17256088
    13. Modeling of corticosteroid effects on hepatic low-density lipoprotein receptors and plasma lipid dynamics in rats. PMID: 17674160

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Membrane, clathrin-coated pit. Golgi apparatus. Early endosome. Late endosome. Lysosome.
  • 蛋白家族：
    LDLR family
  • 数据库链接：

    KEGG: rno:300438

    STRING: 10116.ENSRNOP00000013496

    UniGene: Rn.10483

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