Rat macrophage inflammatory protein 1α,MIP-1α ELISA Kit
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中文名称：大鼠巨噬细胞炎症蛋白1α(MIP-1α/CCL3)酶联免疫试剂盒
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货号：CSB-E07422r-IS
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规格：96T/48T
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价格：￥3200/￥2500
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其他：
产品详情
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产品描述：大鼠巨噬细胞炎症蛋白1α(MIP-1α/CCL3)酶联免疫试剂盒（CSB-E07422r-IS）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CCL3含量。CCL3是一个备受关注的靶点。它在免疫调节等生理过程中发挥重要作用。相关研究聚焦其在炎症、肿瘤等疾病中的机制，探索其与免疫细胞招募、活化的关联，试图通过干预CCL3来调控免疫反应，为疾病治疗提供新策略。试剂盒检测范围为3.9 pg/mL-250 pg/mL，灵敏度为1.95 pg/mL。MIP-1α作为趋化因子家族成员，在炎症反应、免疫细胞趋化及组织修复中发挥关键作用，常用于研究感染性疾病、自身免疫反应及损伤修复等科研场景。科研人员可通过该产品定量分析炎症相关疾病模型中MIP-1α的动态变化，为探索免疫调控机制或评估抗炎药物效果提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：Ccl3 ELISA Kit; Mip1a ELISA Kit; Scya3 ELISA Kit; C-C motif chemokine 3 ELISA Kit; Macrophage inflammatory protein 1-alpha ELISA Kit; MIP-1-alpha ELISA Kit; Small-inducible cytokine A3 ELISA Kit
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缩写：
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Uniprot No.：
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, cell culture supernates, tissue homogenates
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检测范围：3.9 pg/mL-250 pg/mL
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灵敏度：1.95 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
94
Range %
89-99
1:2
Average %
101
Range %
94-108
1:4
Average %
97
Range %
92-102
1:8
Average %
93
Range %
89-98
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回收率：
The recovery of rat MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
91
85-98
EDTA plasma (n=4)
97
90-104
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
250
2.722
2.634
2.678
2.550
125
2.023
2.087
2.055
1.927
62.5
1.236
1.268
1.252
1.124
31.25
0.765
0.729
0.747
0.619
15.6
0.514
0.501
0.507
0.379
7.8
0.286
0.273
0.280
0.152
3.9
0.225
0.214
0.219
0.091
0
0.131
0.125
0.128
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数据处理：
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货期：3-5 working days
产品评价
相关产品
靶点详情
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功能：Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.
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基因功能参考文献：
- The important chemokine macrophage inflammatory protein1alpha increases inflammatory responses and the secretion of inflammatory molecules from Kupffer cells, including tumor necrosis factoralpha, interleukin (IL)1beta and IL6. PMID: 28677732
- The present report demonstrates an increase in mRNA and protein levels in chemokines CCL3 and its receptor CCR5 in dorsal root ganglion in the rat model of remifentanil-induced hyperalgesia. Furthermore, the results pointed out that CCL3 and its receptor CCR5 might be implicated in remifentanil-induced hyperalgesia. PMID: 26550961
- Study shows that erythropoietin reduced chemokine CCL3 mRNA expression in rat cerebellar neuron-enriched preparations during neuroinflammation. PMID: 26178913
- The results of this study suggest a contribution of CCL3 in the spinal dorsal horn to paclitaxel-induced allodynia and may provide new therapeutic targets for paclitaxel-induced painful neuropathy. PMID: 25127716
- Expression levels of CCL3 in the spinal cord are up-regulated following nerve injury. PMID: 24589480
- NMDA-evoked neuronal injury differentially induces MIP-1alpha and MCP-1 production in microglia and astrocytes, respectively, through activation of different intracellular signaling pathways. PMID: 22791363
- Our data suggest that free radicals produced in CA1 neurons contribute to the recruitment and activation of microglia and neurodegeneration through MIP-1alpha expression. PMID: 18272692
- In this study we present data on long term increase in CCL3 mRNA expression and immunoreactivity following epileptogenic stimulus in a rat model of temporal lobe epilepsy. PMID: 21731074
- Data show significant concentration increases for CXCL1 and MIP-1alpha in the brain after soman-induced seizure onset. PMID: 21535896
- The active behavior of the MIP-1alpha gene in KCs following liver ischemia/reperfusion injury is assumed to be one of the major causes for the hepatic ischemia/reperfusion injury. PMID: 16804970
- P2Y6 activation by uridine 5'-diphosphate induces Ccl2 and Ccl3 expression in primary cultured microglia, astrocytes, and brain slices from neonatal and adult rats. PMID: 21317391
- a massive amount of ATP molecules released in the glioma tumor site may act as the regulator with P2XR signaling that increases MIP-1alpha and MCP-1 expression in tumor-infiltrating microglia/macrophages. PMID: 21162127
- These results suggest that MIP-1alpha is directly involved in the neurodegeneration induced in rats by PVC-211 murine leukemia virus by recruiting macrophages/microglia from the periphery into regions of the brain that eventually become diseased. PMID: 21051067
- expression is regulated by nicotine in alveolar macrophages PMID: 12101081
- The constitutive patterns of MIP-1 alpha mRNA and protein expression are demonstrated in rat brain throughout postnatal development within local microenvironments where the postnatal cell migration phenomenon occurs. PMID: 12899200
- Oligodendroglia express the CCL3 receptor CCR1, but not CCR5. CCL3 induced an intracellular rise in Ca(2+), a typical signalling event of G protein-coupled receptors. PMID: 14583384
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亚细胞定位：Secreted.
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蛋白家族：Intercrine beta (chemokine CC) family
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数据库链接：
