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  4. Rat matrix metalloproteinase 13,MMP-13 ELISA kit

Rat matrix metalloproteinase 13,MMP-13 ELISA kit

文献引用 (10) 说明书下载
  • 中文名称：
    大鼠基质金属蛋白酶13(MMP-13)酶联免疫试剂盒
  • 货号：
    CSB-E07412r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠基质金属蛋白酶13(MMP-13)酶联免疫试剂盒（CSB-E07412r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP13含量。MMP13即基质金属蛋白酶 13，在人体组织重塑、胚胎发育等生理过程中发挥作用，异常表达与关节炎、癌症等疾病相关。研究聚焦其在疾病发生发展中的机制，如促进肿瘤侵袭转移等，旨在开发针对性治疗药物。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，适用于探索骨关节炎、肿瘤侵袭转移等疾病模型中基质代谢调控机制，或评估创伤修复、炎症微环境等基础研究中的蛋白酶活性动态本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Mmp13 ELISA Kit; Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit; UMRCASE ELISA Kit; Fragment ELISA Kit
  • 缩写：
    MMP13
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    93.75 pg/mL-6000 pg/mL
  • 灵敏度：
    23.44 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %83-90
    1:2Average %94
    Range %90-98
    1:4Average %98
    Range %93-104
    1:8Average %102
    Range %98-105
  • 回收率：
    The recovery of rat MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
    EDTA plasma (n=4)9085-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    60002.231 2.269 2.250 2.138
    30001.526 1.539 1.533 1.421
    15000.974 0.997 0.986 0.874
    7500.578 0.596 0.587 0.475
    3750.345 0.327 0.336 0.224
    187.50.251 0.241 0.246 0.134
    93.750.185 0.189 0.187 0.075
    00.111 0.113 0.112 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Mmp13 Proteins

靶点详情

  • 功能：
    Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：

    STRING: 10116.ENSRNOP00000011507

    UniGene: Rn.10997

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