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  4. Rat matrix metalloproteinase 3,MMP-3 ELISA kit

Rat matrix metalloproteinase 3,MMP-3 ELISA kit

文献引用 (7) 说明书下载
  • 中文名称：
    大鼠基质金属蛋白酶3(MMP-3)酶联免疫试剂盒
  • 货号：
    CSB-E07410r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠基质金属蛋白酶3(MMP-3)酶联免疫试剂盒（CSB-E07410r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MMP3含量。MMP3，即基质金属蛋白酶3，是一种在多种生理和病理过程中发挥关键作用的酶类分子。它在炎症性疾病、肿瘤、动脉粥样硬化和神经系统疾病中扮演重要角色，能够降解多种基质成分，并激活其他MMPs，参与组织重塑和疾病进程。研究显示，MMP3的基因多态性与某些疾病的遗传易感性相关，如食管鳞癌。试剂盒检测范围为1.56 pg/mL-100 pg/mL，本产品可广泛应用于基础医学研究领域，如关节炎动物模型中的基质代谢研究、药物干预下MMP - 3表达水平动态监测，或肿瘤微环境中蛋白酶活性评估等实验场景，为组织重塑机制探索及病理模型构建提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Mmp3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; PTR1 protein ELISA Kit; Transin-1 ELISA Kit
  • 缩写：
    MMP3
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %90
    Range %87-93
    1:200Average %94
    Range %90-98
    1:400Average %95
    Range %91-99
    1:800Average %92
    Range %88-96
  • 回收率：
    The recovery of rat MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8581-89
    EDTA plasma (n=4)9490-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.569 2.547 2.558 2.429
    502.236 2.213 2.225 2.096
    251.868 1.829 1.849 1.720
    12.51.367 1.387 1.377 1.248
    6.250.840 0.889 0.865 0.736
    3.130.377 0.387 0.382 0.253
    1.560.247 0.259 0.253 0.124
    00.128 0.130 0.129 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Mmp3 Proteins

靶点详情

  • 功能：
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：

    UniGene: Rn.32086

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